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A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds

Tenje, Maria LU ; Cantoni, Federico ; Porras Hernández, Ana María ; Searle, Sean S. ; Johansson, Sofia LU ; Barbe, Laurent ; Antfolk, Maria LU and Pohlit, Hannah (2020) In Organs-on-a-Chip 2.
Abstract
This review article describes microfabrication techniques to define chemical, mechanical and structural patterns in hydrogels and how these can be used to prepare in vivo like, i.e. biomimetic, cell culture scaffolds. Hydrogels are attractive materials for 3D cell cultures as they provide ideal culture conditions and they are becoming more prominently used. Single material gels without any modifications do however have their limitation in use and much can be gained by in improving the in vivo resemblance of simple hydrogel cell culture scaffolds. This review article discusses the most commonly used cross-linking strategies used for hydrogel-based culture scaffolds and gives a brief introduction to microfabrication methods that can be used... (More)
This review article describes microfabrication techniques to define chemical, mechanical and structural patterns in hydrogels and how these can be used to prepare in vivo like, i.e. biomimetic, cell culture scaffolds. Hydrogels are attractive materials for 3D cell cultures as they provide ideal culture conditions and they are becoming more prominently used. Single material gels without any modifications do however have their limitation in use and much can be gained by in improving the in vivo resemblance of simple hydrogel cell culture scaffolds. This review article discusses the most commonly used cross-linking strategies used for hydrogel-based culture scaffolds and gives a brief introduction to microfabrication methods that can be used to define chemical, mechanical and structural patterns in hydrogels with micrometre resolution. The review article also describes a selection of literature references using these microfabrication techniques to prepare organ and disease models with controlled cell adhesion, proliferation and migration. It is intended to serve as an introduction to microfabrication of hydrogels and an inspiration for novel interdisciplinary research projects. (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Organs-on-a-Chip
volume
2
article number
100003
publisher
Elsevier
ISSN
2666-1020
DOI
10.1016/j.ooc.2020.100003
language
English
LU publication?
no
id
d6a959a0-9ea8-4cc7-8c7f-946ed50199bb
date added to LUP
2024-04-04 19:28:16
date last changed
2024-04-05 13:06:05
@article{d6a959a0-9ea8-4cc7-8c7f-946ed50199bb,
  abstract     = {{This review article describes microfabrication techniques to define chemical, mechanical and structural patterns in hydrogels and how these can be used to prepare in vivo like, i.e. biomimetic, cell culture scaffolds. Hydrogels are attractive materials for 3D cell cultures as they provide ideal culture conditions and they are becoming more prominently used. Single material gels without any modifications do however have their limitation in use and much can be gained by in improving the in vivo resemblance of simple hydrogel cell culture scaffolds. This review article discusses the most commonly used cross-linking strategies used for hydrogel-based culture scaffolds and gives a brief introduction to microfabrication methods that can be used to define chemical, mechanical and structural patterns in hydrogels with micrometre resolution. The review article also describes a selection of literature references using these microfabrication techniques to prepare organ and disease models with controlled cell adhesion, proliferation and migration. It is intended to serve as an introduction to microfabrication of hydrogels and an inspiration for novel interdisciplinary research projects.}},
  author       = {{Tenje, Maria and Cantoni, Federico and Porras Hernández, Ana María and Searle, Sean S. and Johansson, Sofia and Barbe, Laurent and Antfolk, Maria and Pohlit, Hannah}},
  issn         = {{2666-1020}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Organs-on-a-Chip}},
  title        = {{A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds}},
  url          = {{http://dx.doi.org/10.1016/j.ooc.2020.100003}},
  doi          = {{10.1016/j.ooc.2020.100003}},
  volume       = {{2}},
  year         = {{2020}},
}