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Identification of a candidate dwarfing gene in Pallas, the first commercial barley cultivar generated through mutational breeding

Zakhrabekova, Shakhira LU ; Chauhan, Pallavi LU ; Dockter, Christoph ; Ealumalai, Pavithra ; Ivanova, Anastasiia ; Jørgensen, Morten Egevang ; Lu, Qiongxian ; Shoeva, Olesya LU ; Werner, Klaudia and Hansson, Mats LU (2023) In Frontiers in Genetics 14.
Abstract

Many induced mutants are available in barley (Hordeum vulgare L.). One of the largest groups of induced mutants is the Erectoides (ert) mutants, which is characterized by a compact and upright spike and a shortened culm. One isolated mutant, ert-k.32, generated by X-ray treatment and registered in 1958 under the named “Pallas”, was the first ever induced barley mutant to be released on the market. Its value was improved culm strength and enhanced lodging resistance. In this study, we aimed to identify the casual gene of the ert-k.32 mutant by whole genome sequencing of allelic ert-k mutants. The suggested Ert-k candidate gene, HORVU.MOREX.r3.6HG0574880, is located in the centromeric region of chromosome 6H. The gene product is an... (More)

Many induced mutants are available in barley (Hordeum vulgare L.). One of the largest groups of induced mutants is the Erectoides (ert) mutants, which is characterized by a compact and upright spike and a shortened culm. One isolated mutant, ert-k.32, generated by X-ray treatment and registered in 1958 under the named “Pallas”, was the first ever induced barley mutant to be released on the market. Its value was improved culm strength and enhanced lodging resistance. In this study, we aimed to identify the casual gene of the ert-k.32 mutant by whole genome sequencing of allelic ert-k mutants. The suggested Ert-k candidate gene, HORVU.MOREX.r3.6HG0574880, is located in the centromeric region of chromosome 6H. The gene product is an alpha/beta hydrolase with a catalytic triad in the active site composed of Ser-167, His-261 and Asp-232. In comparison to proteins derived from the Arabidopsis genome, ErtK is most similar to a thioesterase with de-S-acylation activity. This suggests that ErtK catalyzes post-translational modifications by removing fatty acids that are covalently attached to cysteine residues of target proteins involved in regulation of plant architecture and important commercial traits such as culm stability and lodging resistance.

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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
erectoides, Hordeum vulgare, lodging, semidwarf, spike phenotype
in
Frontiers in Genetics
volume
14
article number
1213815
pages
11 pages
publisher
Frontiers Media S. A.
external identifiers
  • pmid:37470037
  • scopus:85165184749
ISSN
1664-8021
DOI
10.3389/fgene.2023.1213815
language
English
LU publication?
yes
id
d6bdf012-b20d-4467-9b48-5eca9ff87db2
date added to LUP
2023-09-27 11:32:51
date last changed
2024-04-19 01:44:23
@article{d6bdf012-b20d-4467-9b48-5eca9ff87db2,
  abstract     = {{<p>Many induced mutants are available in barley (Hordeum vulgare L.). One of the largest groups of induced mutants is the Erectoides (ert) mutants, which is characterized by a compact and upright spike and a shortened culm. One isolated mutant, ert-k.32, generated by X-ray treatment and registered in 1958 under the named “Pallas”, was the first ever induced barley mutant to be released on the market. Its value was improved culm strength and enhanced lodging resistance. In this study, we aimed to identify the casual gene of the ert-k.32 mutant by whole genome sequencing of allelic ert-k mutants. The suggested Ert-k candidate gene, HORVU.MOREX.r3.6HG0574880, is located in the centromeric region of chromosome 6H. The gene product is an alpha/beta hydrolase with a catalytic triad in the active site composed of Ser-167, His-261 and Asp-232. In comparison to proteins derived from the Arabidopsis genome, ErtK is most similar to a thioesterase with de-S-acylation activity. This suggests that ErtK catalyzes post-translational modifications by removing fatty acids that are covalently attached to cysteine residues of target proteins involved in regulation of plant architecture and important commercial traits such as culm stability and lodging resistance.</p>}},
  author       = {{Zakhrabekova, Shakhira and Chauhan, Pallavi and Dockter, Christoph and Ealumalai, Pavithra and Ivanova, Anastasiia and Jørgensen, Morten Egevang and Lu, Qiongxian and Shoeva, Olesya and Werner, Klaudia and Hansson, Mats}},
  issn         = {{1664-8021}},
  keywords     = {{erectoides; Hordeum vulgare; lodging; semidwarf; spike phenotype}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Genetics}},
  title        = {{Identification of a candidate dwarfing gene in Pallas, the first commercial barley cultivar generated through mutational breeding}},
  url          = {{http://dx.doi.org/10.3389/fgene.2023.1213815}},
  doi          = {{10.3389/fgene.2023.1213815}},
  volume       = {{14}},
  year         = {{2023}},
}