Morphological and ultrastructural alterations of zebrafish (Danio rerio) spermatozoa after motility activation
Sáez-Espinosa, Paula ; Franco-Esclapez, Cristina ; Robles-Gómez, Laura ; Silva, Willian T.A.F. LU
; Romero, Alejandro
; Immler, Simone
and Gómez-Torres, María José
(2022)
In Theriogenology
188.
p.108-115
- Abstract
Spermatozoa motility in freshwater and marine fish is mainly controlled by the difference in osmotic pressure. Specifically, zebrafish (Danio rerio) spermatozoa undergo hypoosmotic shock due to the decrease in extracellular potassium, which leads to membrane hyperpolarization and activation of flagellar motility. Previous studies have concluded that motility activation has a negative effect on the spermatozoa structure. However, no evidence exists about ultrastructural changes in zebrafish spermatozoa after motility activation. In this study, spermatozoa samples were obtained from ten adult zebrafish individuals before and 60 s after motility activation and analyzed using Scanning and Transmission Electron Microscopy. Results showed... (More)
Spermatozoa motility in freshwater and marine fish is mainly controlled by the difference in osmotic pressure. Specifically, zebrafish (Danio rerio) spermatozoa undergo hypoosmotic shock due to the decrease in extracellular potassium, which leads to membrane hyperpolarization and activation of flagellar motility. Previous studies have concluded that motility activation has a negative effect on the spermatozoa structure. However, no evidence exists about ultrastructural changes in zebrafish spermatozoa after motility activation. In this study, spermatozoa samples were obtained from ten adult zebrafish individuals before and 60 s after motility activation and analyzed using Scanning and Transmission Electron Microscopy. Results showed dramatic morphological and ultrastructural alterations of the zebrafish spermatozoa after activation. In particular, the spermatozoa head underwent severe morphological distortion, including swelling of the nucleus, the bursting of the plasma membrane, and the alteration of the genetic material. Midpieces were also affected after activation since rupture of the cell membrane and lysis of mitochondria occurred. Furthermore, after the hypoosmotic shock, most spermatozoa showed a coiled flagellum and a disaggregated plasma membrane. Overall, our findings show that the activation of motility leads to substantial zebrafish spermatozoa morphological and ultrastructural changes, which could modify their physiology and decrease the fertilizing potential.
(Less)
- author
- Sáez-Espinosa, Paula
; Franco-Esclapez, Cristina
; Robles-Gómez, Laura
; Silva, Willian T.A.F.
LU
; Romero, Alejandro
; Immler, Simone
and Gómez-Torres, María José
- organization
- publishing date
- 2022-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Motility activation, Sperm morphology, Ultrastructure microscopy, Zebrafish
- in
- Theriogenology
- volume
- 188
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85131520179
- pmid:35688040
- ISSN
- 0093-691X
- DOI
- 10.1016/j.theriogenology.2022.05.025
- language
- English
- LU publication?
- yes
- id
- d6be4beb-7f69-4412-8f62-f73e5cca4665
- date added to LUP
- 2022-08-19 17:02:50
- date last changed
- 2024-04-18 05:19:40
@article{d6be4beb-7f69-4412-8f62-f73e5cca4665,
abstract = {{<p>Spermatozoa motility in freshwater and marine fish is mainly controlled by the difference in osmotic pressure. Specifically, zebrafish (Danio rerio) spermatozoa undergo hypoosmotic shock due to the decrease in extracellular potassium, which leads to membrane hyperpolarization and activation of flagellar motility. Previous studies have concluded that motility activation has a negative effect on the spermatozoa structure. However, no evidence exists about ultrastructural changes in zebrafish spermatozoa after motility activation. In this study, spermatozoa samples were obtained from ten adult zebrafish individuals before and 60 s after motility activation and analyzed using Scanning and Transmission Electron Microscopy. Results showed dramatic morphological and ultrastructural alterations of the zebrafish spermatozoa after activation. In particular, the spermatozoa head underwent severe morphological distortion, including swelling of the nucleus, the bursting of the plasma membrane, and the alteration of the genetic material. Midpieces were also affected after activation since rupture of the cell membrane and lysis of mitochondria occurred. Furthermore, after the hypoosmotic shock, most spermatozoa showed a coiled flagellum and a disaggregated plasma membrane. Overall, our findings show that the activation of motility leads to substantial zebrafish spermatozoa morphological and ultrastructural changes, which could modify their physiology and decrease the fertilizing potential.</p>}},
author = {{Sáez-Espinosa, Paula and Franco-Esclapez, Cristina and Robles-Gómez, Laura and Silva, Willian T.A.F. and Romero, Alejandro and Immler, Simone and Gómez-Torres, María José}},
issn = {{0093-691X}},
keywords = {{Motility activation; Sperm morphology; Ultrastructure microscopy; Zebrafish}},
language = {{eng}},
pages = {{108--115}},
publisher = {{Elsevier}},
series = {{Theriogenology}},
title = {{Morphological and ultrastructural alterations of zebrafish (Danio rerio) spermatozoa after motility activation}},
url = {{http://dx.doi.org/10.1016/j.theriogenology.2022.05.025}},
doi = {{10.1016/j.theriogenology.2022.05.025}},
volume = {{188}},
year = {{2022}},
}