Proteomics profiling of human synovial fluid suggests increased protein interplay in early-osteoarthritis (OA) that is lost in late-stage OA
Ali, Neserin LU
; Turkiewicz, Aleksandra
LU
; Hughes, Velocity
LU
; Folkesson, Elin
; Tjörnstand, Jon
LU
; Neuman, Paul
LU
; Önnerfjord, Patrik
LU
and Englund, Martin
LU
(2022)
In Molecular & Cellular Proteomics
- Abstract
- The underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA.
Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e.... (More) - The underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA.
Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e. interplay) between the proteins in each group.
More proteins were increased in early-OA vs controls than late-stage OA vs controls. For most of these proteins, the fold changes between late-stage OA vs controls and early stage OA vs controls were remarkably similar suggesting potential involvement in the OA process. Further, for the first time this study illustrated distinct patterns in protein co-expression suggesting that the interplay between the protein machinery is increased in early-OA and lost in late-stage OA. Further efforts should focus on earlier stages of the disease than previously considered. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d8024943-072c-4e58-900b-67f0e8120805
- author
- Ali, Neserin
LU
; Turkiewicz, Aleksandra
LU
; Hughes, Velocity
LU
; Folkesson, Elin
; Tjörnstand, Jon
LU
; Neuman, Paul
LU
; Önnerfjord, Patrik
LU
and Englund, Martin
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular & Cellular Proteomics
- article number
- 100200
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:35074580
- scopus:85127116304
- ISSN
- 1535-9484
- DOI
- 10.1016/j.mcpro.2022.100200
- language
- English
- LU publication?
- yes
- id
- d8024943-072c-4e58-900b-67f0e8120805
- date added to LUP
- 2022-01-31 01:08:21
- date last changed
- 2022-05-06 04:12:39
@article{d8024943-072c-4e58-900b-67f0e8120805,
abstract = {{The underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA.<br/><br/>Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e. interplay) between the proteins in each group.<br/><br/>More proteins were increased in early-OA vs controls than late-stage OA vs controls. For most of these proteins, the fold changes between late-stage OA vs controls and early stage OA vs controls were remarkably similar suggesting potential involvement in the OA process. Further, for the first time this study illustrated distinct patterns in protein co-expression suggesting that the interplay between the protein machinery is increased in early-OA and lost in late-stage OA. Further efforts should focus on earlier stages of the disease than previously considered.}},
author = {{Ali, Neserin and Turkiewicz, Aleksandra and Hughes, Velocity and Folkesson, Elin and Tjörnstand, Jon and Neuman, Paul and Önnerfjord, Patrik and Englund, Martin}},
issn = {{1535-9484}},
language = {{eng}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Molecular & Cellular Proteomics}},
title = {{Proteomics profiling of human synovial fluid suggests increased protein interplay in early-osteoarthritis (OA) that is lost in late-stage OA}},
url = {{http://dx.doi.org/10.1016/j.mcpro.2022.100200}},
doi = {{10.1016/j.mcpro.2022.100200}},
year = {{2022}},
}