Impact of monocytic cells on recovery of uncultivable bacteria from atherosclerotic lesions
(2011) In Journal of Internal Medicine 270(3). p.273-280- Abstract
Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon... (More)
Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon signed-rank tests were used to determine whether THP-1 treatment yielded a higher number of isolates than did the untreated controls. Results. We recovered more bacteria from cocultures of atherectomy specimens with THP-1 cells than atherectomy specimens cultured alone. On average, tissue homogenates incubated with THP-1 cells versus control yielded 124 vs. 22 colony-forming units, a median of 140 vs. 7, respectively (P=0.02). We recovered 872 isolates of limited number of species, including Propionibacterium acnes, Staphylococcus epidermidis and Streptococcus infantis and the fastidious anaerobe Porphyromonas gingivalis, and confirmed its presence in tissue using double immunofluorescence imaging. qPCR demonstrated the presence of ≥3.5×103P. gingivalis genomes per gram of atheromatous tissue. Conclusions. These results indicate that viable previously uncultivable bacterial species are present within atheromas. Our results suggest revisiting the hypothesis that infections may have a causative role in atherosclerotic inflammation and have implications for research regarding novel diagnostics and treatments for cardiovascular disease.
(Less)
- author
- Rafferty, B. ; Jönsson, D. LU ; Kalachikov, S. ; Demmer, R. T. ; Nowygrod, R. ; Elkind, M. S.V. ; Bush, H. and Kozarov, E.
- publishing date
- 2011-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Atherosclerosis, Bacterial infection, Monocytes, Periodontal disease, Porphyromonas gingivalis
- in
- Journal of Internal Medicine
- volume
- 270
- issue
- 3
- pages
- 8 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:80051553681
- pmid:21366733
- ISSN
- 0954-6820
- DOI
- 10.1111/j.1365-2796.2011.02373.x
- language
- English
- LU publication?
- no
- id
- d83587de-266c-4d34-942a-4c4bb757369e
- date added to LUP
- 2020-12-04 13:41:21
- date last changed
- 2024-04-17 22:52:04
@article{d83587de-266c-4d34-942a-4c4bb757369e,
abstract = {{<p>Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon signed-rank tests were used to determine whether THP-1 treatment yielded a higher number of isolates than did the untreated controls. Results. We recovered more bacteria from cocultures of atherectomy specimens with THP-1 cells than atherectomy specimens cultured alone. On average, tissue homogenates incubated with THP-1 cells versus control yielded 124 vs. 22 colony-forming units, a median of 140 vs. 7, respectively (P=0.02). We recovered 872 isolates of limited number of species, including Propionibacterium acnes, Staphylococcus epidermidis and Streptococcus infantis and the fastidious anaerobe Porphyromonas gingivalis, and confirmed its presence in tissue using double immunofluorescence imaging. qPCR demonstrated the presence of ≥3.5×10<sup>3</sup>P. gingivalis genomes per gram of atheromatous tissue. Conclusions. These results indicate that viable previously uncultivable bacterial species are present within atheromas. Our results suggest revisiting the hypothesis that infections may have a causative role in atherosclerotic inflammation and have implications for research regarding novel diagnostics and treatments for cardiovascular disease.</p>}},
author = {{Rafferty, B. and Jönsson, D. and Kalachikov, S. and Demmer, R. T. and Nowygrod, R. and Elkind, M. S.V. and Bush, H. and Kozarov, E.}},
issn = {{0954-6820}},
keywords = {{Atherosclerosis; Bacterial infection; Monocytes; Periodontal disease; Porphyromonas gingivalis}},
language = {{eng}},
number = {{3}},
pages = {{273--280}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Internal Medicine}},
title = {{Impact of monocytic cells on recovery of uncultivable bacteria from atherosclerotic lesions}},
url = {{http://dx.doi.org/10.1111/j.1365-2796.2011.02373.x}},
doi = {{10.1111/j.1365-2796.2011.02373.x}},
volume = {{270}},
year = {{2011}},
}