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Impact of monocytic cells on recovery of uncultivable bacteria from atherosclerotic lesions

Rafferty, B. ; Jönsson, D. LU ; Kalachikov, S. ; Demmer, R. T. ; Nowygrod, R. ; Elkind, M. S.V. ; Bush, H. and Kozarov, E. (2011) In Journal of Internal Medicine 270(3). p.273-280
Abstract

Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon... (More)

Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon signed-rank tests were used to determine whether THP-1 treatment yielded a higher number of isolates than did the untreated controls. Results. We recovered more bacteria from cocultures of atherectomy specimens with THP-1 cells than atherectomy specimens cultured alone. On average, tissue homogenates incubated with THP-1 cells versus control yielded 124 vs. 22 colony-forming units, a median of 140 vs. 7, respectively (P=0.02). We recovered 872 isolates of limited number of species, including Propionibacterium acnes, Staphylococcus epidermidis and Streptococcus infantis and the fastidious anaerobe Porphyromonas gingivalis, and confirmed its presence in tissue using double immunofluorescence imaging. qPCR demonstrated the presence of ≥3.5×103P. gingivalis genomes per gram of atheromatous tissue. Conclusions. These results indicate that viable previously uncultivable bacterial species are present within atheromas. Our results suggest revisiting the hypothesis that infections may have a causative role in atherosclerotic inflammation and have implications for research regarding novel diagnostics and treatments for cardiovascular disease.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Atherosclerosis, Bacterial infection, Monocytes, Periodontal disease, Porphyromonas gingivalis
in
Journal of Internal Medicine
volume
270
issue
3
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:21366733
  • scopus:80051553681
ISSN
0954-6820
DOI
10.1111/j.1365-2796.2011.02373.x
language
English
LU publication?
no
id
d83587de-266c-4d34-942a-4c4bb757369e
date added to LUP
2020-12-04 13:41:21
date last changed
2024-05-01 23:15:56
@article{d83587de-266c-4d34-942a-4c4bb757369e,
  abstract     = {{<p>Objective. Epidemiological evidence suggests that infections may contribute to atherogenesis. However, with the exception of Chlamydophila pneumoniae, cultivable bacteria have not been recovered from atherosclerotic lesions. Therefore, we aimed at developing an approach to recover uncultivable bacteria from atherectomy tissues. Methods. We cultured homogenates from atherectomy specimens from seven nonseptic patients undergoing surgery for arterial obstruction either alone or together with THP-1 monocyte-like cells. We performed 16S rDNA analysis, biochemical tests, random amplification of polymorphic DNA PCR analysis, quantitative polymerase chain reaction (qPCR) and immunohistofluorescence to identify the cultivated bacteria. Wilcoxon signed-rank tests were used to determine whether THP-1 treatment yielded a higher number of isolates than did the untreated controls. Results. We recovered more bacteria from cocultures of atherectomy specimens with THP-1 cells than atherectomy specimens cultured alone. On average, tissue homogenates incubated with THP-1 cells versus control yielded 124 vs. 22 colony-forming units, a median of 140 vs. 7, respectively (P=0.02). We recovered 872 isolates of limited number of species, including Propionibacterium acnes, Staphylococcus epidermidis and Streptococcus infantis and the fastidious anaerobe Porphyromonas gingivalis, and confirmed its presence in tissue using double immunofluorescence imaging. qPCR demonstrated the presence of ≥3.5×10<sup>3</sup>P. gingivalis genomes per gram of atheromatous tissue. Conclusions. These results indicate that viable previously uncultivable bacterial species are present within atheromas. Our results suggest revisiting the hypothesis that infections may have a causative role in atherosclerotic inflammation and have implications for research regarding novel diagnostics and treatments for cardiovascular disease.</p>}},
  author       = {{Rafferty, B. and Jönsson, D. and Kalachikov, S. and Demmer, R. T. and Nowygrod, R. and Elkind, M. S.V. and Bush, H. and Kozarov, E.}},
  issn         = {{0954-6820}},
  keywords     = {{Atherosclerosis; Bacterial infection; Monocytes; Periodontal disease; Porphyromonas gingivalis}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{273--280}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Internal Medicine}},
  title        = {{Impact of monocytic cells on recovery of uncultivable bacteria from atherosclerotic lesions}},
  url          = {{http://dx.doi.org/10.1111/j.1365-2796.2011.02373.x}},
  doi          = {{10.1111/j.1365-2796.2011.02373.x}},
  volume       = {{270}},
  year         = {{2011}},
}