Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography
(2006) In Biotechnology and Bioengineering 93(4). p.656-664- Abstract
- A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extra-column dispersion, the dispersion due to the packed bed, the external mass transfer from the bulk phase to the stationary phase, and the diffusive transport within the stationary phase. The methodology has several advantages compared to previously reported methods to determine diffusion coefficients in that no other equipment than an HPLC is required, any class of stationary phases can be investigated as long as the... (More)
- A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extra-column dispersion, the dispersion due to the packed bed, the external mass transfer from the bulk phase to the stationary phase, and the diffusive transport within the stationary phase. The methodology has several advantages compared to previously reported methods to determine diffusion coefficients in that no other equipment than an HPLC is required, any class of stationary phases can be investigated as long as the experiments are performed under non-binding conditions, and no modification, e.g., moulding of slabs or membranes, to the stationary phase is required. To show the applicability of the methodology, the effective diffusion coefficients of lysozyme, bovine serum albumin, and immunoglobulin gamma in Sepharose (TM) CL-4B were determined and shown to be comparable with those determined with other methods. (c) 2005 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/417230
- author
- Kempe, Henrik LU ; Persson, Patrik LU ; Axelsson, Anders LU ; Nilsson, Bernt LU and Zacchi, Guido LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- effective diffusion coefficient, diffusion, chromatography, frontal, protein separation, downstream processing
- in
- Biotechnology and Bioengineering
- volume
- 93
- issue
- 4
- pages
- 656 - 664
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000235630600006
- pmid:16372360
- scopus:33644913140
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.20738
- language
- English
- LU publication?
- yes
- id
- d896b7e7-c80a-4159-9436-fa5b5f171529 (old id 417230)
- date added to LUP
- 2016-04-01 11:36:32
- date last changed
- 2023-09-01 00:11:43
@article{d896b7e7-c80a-4159-9436-fa5b5f171529,
abstract = {{A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extra-column dispersion, the dispersion due to the packed bed, the external mass transfer from the bulk phase to the stationary phase, and the diffusive transport within the stationary phase. The methodology has several advantages compared to previously reported methods to determine diffusion coefficients in that no other equipment than an HPLC is required, any class of stationary phases can be investigated as long as the experiments are performed under non-binding conditions, and no modification, e.g., moulding of slabs or membranes, to the stationary phase is required. To show the applicability of the methodology, the effective diffusion coefficients of lysozyme, bovine serum albumin, and immunoglobulin gamma in Sepharose (TM) CL-4B were determined and shown to be comparable with those determined with other methods. (c) 2005 Wiley Periodicals, Inc.}},
author = {{Kempe, Henrik and Persson, Patrik and Axelsson, Anders and Nilsson, Bernt and Zacchi, Guido}},
issn = {{1097-0290}},
keywords = {{effective diffusion coefficient; diffusion; chromatography; frontal; protein separation; downstream processing}},
language = {{eng}},
number = {{4}},
pages = {{656--664}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography}},
url = {{http://dx.doi.org/10.1002/bit.20738}},
doi = {{10.1002/bit.20738}},
volume = {{93}},
year = {{2006}},
}