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Evaluation of Laminaria Digitata Hydrolysate for the Production of Bioethanol and Butanol by Fermentation

Moenaert, Antoine ; López-Contreras, Ana M. ; Budde, Miriam ; Allahgholi, Leila LU ; Hou, Xiaoru ; Bjerre, Anne Belinda ; Örlygsson, Johann ; Karlsson, Eva Nordberg LU orcid ; Friðjónsson, Ólafur H. and Hreggviðsson, Guðmundur Óli (2023) In Fermentation 9(1).
Abstract

Seaweeds (macroalgae) are gaining attention as potential sustainable feedstock for the production of fuels and chemicals. This comparative study focuses on the characterization of the microbial production of alcohols from fermentable carbohydrates in the hydrolysate of the macroalgae Laminaria digitata as raw material. The potential of a hydrolysate as a carbon source for the production of selected alcohols was tested, using three physiologically different fermentative microbes, in two main types of processes. For the production of ethanol, Saccharomyces cerevisiae was used as a benchmark microorganism and compared with the strictly anaerobic thermophile Thermoanaerobacterium strain AK17. For mixed production of acetone/isopropanol,... (More)

Seaweeds (macroalgae) are gaining attention as potential sustainable feedstock for the production of fuels and chemicals. This comparative study focuses on the characterization of the microbial production of alcohols from fermentable carbohydrates in the hydrolysate of the macroalgae Laminaria digitata as raw material. The potential of a hydrolysate as a carbon source for the production of selected alcohols was tested, using three physiologically different fermentative microbes, in two main types of processes. For the production of ethanol, Saccharomyces cerevisiae was used as a benchmark microorganism and compared with the strictly anaerobic thermophile Thermoanaerobacterium strain AK17. For mixed production of acetone/isopropanol, butanol, and ethanol (A/IBE), three strictly anaerobic Clostridium strains were compared. All strains grew well on the hydrolysate, and toxicity constraints were not observed, but fermentation performance and product profiles were shown to be both condition- and strain-specific. S. cerevisiae utilized only glucose for ethanol formation, while strain AK17 utilized glucose, mannitol, and parts of the glucan oligosaccharides. The clostridia strains tested showed different nutrient requirements, and were able to utilize glucan, mannitol, and organic acids in the hydrolysate. The novelty of this study embodies the application of different inoculates for fermenting a common brown seaweed found in the northern Atlantic Ocean. It provides important information on the fermentation properties of different microorganisms and pinpoints the value of carbon source utilization when selecting microbes for efficient bioconversion into biofuel and chemical products of interest.

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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ABE fermentation, biobutanol, bioethanol, biorefinery, laminarin, macroalgae, seaweed
in
Fermentation
volume
9
issue
1
article number
59
publisher
MDPI AG
external identifiers
  • scopus:85146804494
ISSN
2311-5637
DOI
10.3390/fermentation9010059
language
English
LU publication?
yes
id
d8acd223-3703-4c45-a17d-f24ad4b86fba
date added to LUP
2023-02-13 13:26:44
date last changed
2023-02-13 13:26:44
@article{d8acd223-3703-4c45-a17d-f24ad4b86fba,
  abstract     = {{<p>Seaweeds (macroalgae) are gaining attention as potential sustainable feedstock for the production of fuels and chemicals. This comparative study focuses on the characterization of the microbial production of alcohols from fermentable carbohydrates in the hydrolysate of the macroalgae Laminaria digitata as raw material. The potential of a hydrolysate as a carbon source for the production of selected alcohols was tested, using three physiologically different fermentative microbes, in two main types of processes. For the production of ethanol, Saccharomyces cerevisiae was used as a benchmark microorganism and compared with the strictly anaerobic thermophile Thermoanaerobacterium strain AK17. For mixed production of acetone/isopropanol, butanol, and ethanol (A/IBE), three strictly anaerobic Clostridium strains were compared. All strains grew well on the hydrolysate, and toxicity constraints were not observed, but fermentation performance and product profiles were shown to be both condition- and strain-specific. S. cerevisiae utilized only glucose for ethanol formation, while strain AK17 utilized glucose, mannitol, and parts of the glucan oligosaccharides. The clostridia strains tested showed different nutrient requirements, and were able to utilize glucan, mannitol, and organic acids in the hydrolysate. The novelty of this study embodies the application of different inoculates for fermenting a common brown seaweed found in the northern Atlantic Ocean. It provides important information on the fermentation properties of different microorganisms and pinpoints the value of carbon source utilization when selecting microbes for efficient bioconversion into biofuel and chemical products of interest.</p>}},
  author       = {{Moenaert, Antoine and López-Contreras, Ana M. and Budde, Miriam and Allahgholi, Leila and Hou, Xiaoru and Bjerre, Anne Belinda and Örlygsson, Johann and Karlsson, Eva Nordberg and Friðjónsson, Ólafur H. and Hreggviðsson, Guðmundur Óli}},
  issn         = {{2311-5637}},
  keywords     = {{ABE fermentation; biobutanol; bioethanol; biorefinery; laminarin; macroalgae; seaweed}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Fermentation}},
  title        = {{Evaluation of Laminaria Digitata Hydrolysate for the Production of Bioethanol and Butanol by Fermentation}},
  url          = {{http://dx.doi.org/10.3390/fermentation9010059}},
  doi          = {{10.3390/fermentation9010059}},
  volume       = {{9}},
  year         = {{2023}},
}