Visualization in freeze-dried tissue sections of structures to be studied by quantitative histochemistry.
(1971) In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 19(3). p.186-191- Abstract
- The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d8afae36-2b8c-4367-83bc-294a1267202f
- author
- Hellman, B. and Lernmark, A. LU
- publishing date
- 1971-01-01
- type
- Contribution to journal
- publication status
- published
- in
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- volume
- 19
- issue
- 3
- pages
- 186 - 191
- publisher
- Histochemical Society
- external identifiers
-
- scopus:0015026351
- pmid:4100997
- ISSN
- 0022-1554
- DOI
- 10.1177/19.3.186
- language
- English
- LU publication?
- no
- id
- d8afae36-2b8c-4367-83bc-294a1267202f
- date added to LUP
- 2019-09-18 12:25:55
- date last changed
- 2024-03-13 08:10:03
@article{d8afae36-2b8c-4367-83bc-294a1267202f, abstract = {{The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.}}, author = {{Hellman, B. and Lernmark, A.}}, issn = {{0022-1554}}, language = {{eng}}, month = {{01}}, number = {{3}}, pages = {{186--191}}, publisher = {{Histochemical Society}}, series = {{The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}}, title = {{Visualization in freeze-dried tissue sections of structures to be studied by quantitative histochemistry.}}, url = {{http://dx.doi.org/10.1177/19.3.186}}, doi = {{10.1177/19.3.186}}, volume = {{19}}, year = {{1971}}, }