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Visualization in freeze-dried tissue sections of structures to be studied by quantitative histochemistry.

Hellman, B. and Lernmark, A. LU orcid (1971) In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 19(3). p.186-191
Abstract
The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.
Please use this url to cite or link to this publication:
author
and
publishing date
type
Contribution to journal
publication status
published
in
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
volume
19
issue
3
pages
186 - 191
publisher
Histochemical Society
external identifiers
  • scopus:0015026351
  • pmid:4100997
ISSN
0022-1554
DOI
10.1177/19.3.186
language
English
LU publication?
no
id
d8afae36-2b8c-4367-83bc-294a1267202f
date added to LUP
2019-09-18 12:25:55
date last changed
2024-03-13 08:10:03
@article{d8afae36-2b8c-4367-83bc-294a1267202f,
  abstract     = {{The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.}},
  author       = {{Hellman, B. and Lernmark, A.}},
  issn         = {{0022-1554}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{186--191}},
  publisher    = {{Histochemical Society}},
  series       = {{The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}},
  title        = {{Visualization in freeze-dried tissue sections of structures to be studied by quantitative histochemistry.}},
  url          = {{http://dx.doi.org/10.1177/19.3.186}},
  doi          = {{10.1177/19.3.186}},
  volume       = {{19}},
  year         = {{1971}},
}