Kayseri Yöresinden Toplanmış Culex pipiens Komplekse ait Sivrisinek (Diptera: Culicidae) Örneklerinin Kan Beslenme İdentifikasyonu
(2016) In Turkiye parazitolojii dergisi 40(4). p.199-204- Abstract
OBJECTIVE: This study aimed to determine the host preferences in blood meal of specimens belonging to Culex pipiens complex.
METHODS: A total of 1284 female mosquitos were morphologically examined, and genomic DNA isolations were individually performed on 376 (28.4%) specimens that were determined to be Cx. pipiens complex. PCR was performed with primers to specifically amplify the avian and mammalian mitochondrial cytochrome b (mt-cytb) gene region. Amplicons were cloned, and the obtained plasmids were sequenced to determine host species.
RESULTS: Of 376 specimens, 148 (39.4%) were positive for the avian and/or mammalian blood meal. Among the positive specimens, 43, 98, and seven were determined to be positive for only... (More)
OBJECTIVE: This study aimed to determine the host preferences in blood meal of specimens belonging to Culex pipiens complex.
METHODS: A total of 1284 female mosquitos were morphologically examined, and genomic DNA isolations were individually performed on 376 (28.4%) specimens that were determined to be Cx. pipiens complex. PCR was performed with primers to specifically amplify the avian and mammalian mitochondrial cytochrome b (mt-cytb) gene region. Amplicons were cloned, and the obtained plasmids were sequenced to determine host species.
RESULTS: Of 376 specimens, 148 (39.4%) were positive for the avian and/or mammalian blood meal. Among the positive specimens, 43, 98, and seven were determined to be positive for only mammalian, avian, and both avian and mammalian blood, respectively. Avian host preference in blood meal of the specimens belonging to Cx. pipiens was found to be significant. Of 15 avian blood positive isolates, nine, three, two, and one were designated as blood meal from avian species in Passeriformes, Accipitriformes, Columbiformes, and Strigiformes orders, respectively. While six, four, three, and two out of 15 mammalian blood-positive specimens were found to be positive for human, cattle, sheep, and dog blood, respectively.
CONCLUSION: Molecular data regarding the host preferences of the Cx. pipiens species complex in blood meal were revealed for the first time in Turkey with this study.
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- author
- Korkmaz, Seval ; Yıldırım, Alparslan ; Düzlü, Önder ; Çiloğlu, Arif LU ; Önder, Zuhal and İnci, Abdullah
- alternative title
- Blood Meal Identification of the Mosquito (Diptera: Culicidae) Specimens Belong to Culex pipiens Complex that were Collected from Kayseri Province
- publishing date
- 2016-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Culex pipiens complex, blood meal, avian, mammalian, Kayseri
- in
- Turkiye parazitolojii dergisi
- volume
- 40
- issue
- 4
- pages
- 6 pages
- publisher
- Aves Yayincilik Ltd.
- external identifiers
-
- scopus:85016409161
- pmid:28091388
- DOI
- 10.5152/tpd.2016.4882
- language
- Turkish
- LU publication?
- no
- id
- d8e7dfc5-e3a4-446b-836c-04af14ae3323
- date added to LUP
- 2017-12-04 15:13:42
- date last changed
- 2024-03-01 10:35:10
@article{d8e7dfc5-e3a4-446b-836c-04af14ae3323,
abstract = {{<p>OBJECTIVE: This study aimed to determine the host preferences in blood meal of specimens belonging to Culex pipiens complex.</p><p>METHODS: A total of 1284 female mosquitos were morphologically examined, and genomic DNA isolations were individually performed on 376 (28.4%) specimens that were determined to be Cx. pipiens complex. PCR was performed with primers to specifically amplify the avian and mammalian mitochondrial cytochrome b (mt-cytb) gene region. Amplicons were cloned, and the obtained plasmids were sequenced to determine host species.</p><p>RESULTS: Of 376 specimens, 148 (39.4%) were positive for the avian and/or mammalian blood meal. Among the positive specimens, 43, 98, and seven were determined to be positive for only mammalian, avian, and both avian and mammalian blood, respectively. Avian host preference in blood meal of the specimens belonging to Cx. pipiens was found to be significant. Of 15 avian blood positive isolates, nine, three, two, and one were designated as blood meal from avian species in Passeriformes, Accipitriformes, Columbiformes, and Strigiformes orders, respectively. While six, four, three, and two out of 15 mammalian blood-positive specimens were found to be positive for human, cattle, sheep, and dog blood, respectively.</p><p>CONCLUSION: Molecular data regarding the host preferences of the Cx. pipiens species complex in blood meal were revealed for the first time in Turkey with this study.</p>}},
author = {{Korkmaz, Seval and Yıldırım, Alparslan and Düzlü, Önder and Çiloğlu, Arif and Önder, Zuhal and İnci, Abdullah}},
keywords = {{Culex pipiens complex; blood meal; avian; mammalian; Kayseri}},
language = {{tur}},
month = {{12}},
number = {{4}},
pages = {{199--204}},
publisher = {{Aves Yayincilik Ltd.}},
series = {{Turkiye parazitolojii dergisi}},
title = {{Kayseri Yöresinden Toplanmış Culex pipiens Komplekse ait Sivrisinek (Diptera: Culicidae) Örneklerinin Kan Beslenme İdentifikasyonu}},
url = {{http://dx.doi.org/10.5152/tpd.2016.4882}},
doi = {{10.5152/tpd.2016.4882}},
volume = {{40}},
year = {{2016}},
}