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Cryogels with affinity ligands as tools in protein purification

Hajizadeh, Solmaz LU orcid and Mattiasson, Bo LU (2015) In Methods in Molecular Biology p.183-183
Abstract
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Affinity Chromatography
series title
Methods in Molecular Biology
editor
Reichelt, Senta
pages
200 pages
publisher
Humana Press
external identifiers
  • scopus:84924566002
ISSN
1940-6029
ISBN
978-1-4939-2446-2
978-1-4939-2447-9
DOI
10.1007/978-1-4939-2447-9_15
language
English
LU publication?
yes
id
d972faba-9433-4e50-91e9-8c51675d8cd9
date added to LUP
2021-11-09 08:49:26
date last changed
2024-01-05 20:01:18
@inbook{d972faba-9433-4e50-91e9-8c51675d8cd9,
  abstract     = {{Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.}},
  author       = {{Hajizadeh, Solmaz and Mattiasson, Bo}},
  booktitle    = {{Affinity Chromatography}},
  editor       = {{Reichelt, Senta}},
  isbn         = {{978-1-4939-2446-2}},
  issn         = {{1940-6029}},
  language     = {{eng}},
  pages        = {{183--183}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Cryogels with affinity ligands as tools in protein purification}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-2447-9_15}},
  doi          = {{10.1007/978-1-4939-2447-9_15}},
  year         = {{2015}},
}