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Subcellular distribution and autophosphorylation of calcium/calmodulin- dependent protein kinase II-α in rat hippocampus in a model of ischemic tolerance

Shamloo, M. LU ; Kamme, F. LU and Wieloch, Tadeusz LU (2000) In Neuroscience 96(4). p.665-674
Abstract

A brief period of sublethal ischemia induces resistance to a subsequent, otherwise lethal, ischemic insult, a process named ischemic tolerance or preconditioning. A persistently disturbed cell signaling during reperfusion after cerebral ischemia has been proposed to contribute to ischemic cell death. Here, we report on the effect of ischemic preconditioning on the levels of the regulatory α-subunit of calcium/calmodulin protein kinase II and its phosphorylation in the hippocampal CA1 region. We found that during and following lethal cerebral ischemia, calcium/calmodulin protein kinase II- α is persistently translocated to cell membranes, where it becomes phosphorylated at threonine 286. In contrast, in the preconditioned brains the... (More)

A brief period of sublethal ischemia induces resistance to a subsequent, otherwise lethal, ischemic insult, a process named ischemic tolerance or preconditioning. A persistently disturbed cell signaling during reperfusion after cerebral ischemia has been proposed to contribute to ischemic cell death. Here, we report on the effect of ischemic preconditioning on the levels of the regulatory α-subunit of calcium/calmodulin protein kinase II and its phosphorylation in the hippocampal CA1 region. We found that during and following lethal cerebral ischemia, calcium/calmodulin protein kinase II- α is persistently translocated to cell membranes, where it becomes phosphorylated at threonine 286. In contrast, in the preconditioned brains the translocation and phosphorylation are transient and return to preischemic values after one day of reperfusion. At this time of reperfusion, the total level of calcium/calmodulin protein kinase II-α is significantly lower in preconditioned animals compared to the sham and non-conditioned animals. After one day of reperfusion, the level of calcium/calmodulin protein kinase II-α messenger RNA decreases in the non-conditioned brains, whereas it is unchanged in preconditioned brains. We conclude that, during and after ischemia, calcium/calmodulin protein kinase II-α is translocated to cell membranes and becomes phosphorylated at threonine 286. This could detrimentally influence cell survival by changing receptor function and ion channel conductance. Ischemic preconditioning prevents the persistent presence of calcium/calmodulin protein kinase II-α at cell membranes, presumably by enhancing its degradation, which could be part of a neuroprotective mechanism of ischemic tolerance. (C) 2000 IBRO.

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type
Contribution to journal
publication status
published
subject
keywords
Brain ischemia, Calcium/calmodulin-dependent protein kinase II, Cell death, Phosphorylation, Tolerance
in
Neuroscience
volume
96
issue
4
pages
10 pages
publisher
Elsevier
external identifiers
  • Scopus:0034112048
ISSN
0306-4522
DOI
10.1016/S0306-4522(99)00586-2
language
English
LU publication?
yes
id
d99357b0-4607-44f6-8571-81e67dfa382b
date added to LUP
2016-10-05 19:00:08
date last changed
2017-01-01 08:36:05
@article{d99357b0-4607-44f6-8571-81e67dfa382b,
  abstract     = {<p>A brief period of sublethal ischemia induces resistance to a subsequent, otherwise lethal, ischemic insult, a process named ischemic tolerance or preconditioning. A persistently disturbed cell signaling during reperfusion after cerebral ischemia has been proposed to contribute to ischemic cell death. Here, we report on the effect of ischemic preconditioning on the levels of the regulatory α-subunit of calcium/calmodulin protein kinase II and its phosphorylation in the hippocampal CA1 region. We found that during and following lethal cerebral ischemia, calcium/calmodulin protein kinase II- α is persistently translocated to cell membranes, where it becomes phosphorylated at threonine 286. In contrast, in the preconditioned brains the translocation and phosphorylation are transient and return to preischemic values after one day of reperfusion. At this time of reperfusion, the total level of calcium/calmodulin protein kinase II-α is significantly lower in preconditioned animals compared to the sham and non-conditioned animals. After one day of reperfusion, the level of calcium/calmodulin protein kinase II-α messenger RNA decreases in the non-conditioned brains, whereas it is unchanged in preconditioned brains. We conclude that, during and after ischemia, calcium/calmodulin protein kinase II-α is translocated to cell membranes and becomes phosphorylated at threonine 286. This could detrimentally influence cell survival by changing receptor function and ion channel conductance. Ischemic preconditioning prevents the persistent presence of calcium/calmodulin protein kinase II-α at cell membranes, presumably by enhancing its degradation, which could be part of a neuroprotective mechanism of ischemic tolerance. (C) 2000 IBRO.</p>},
  author       = {Shamloo, M. and Kamme, F. and Wieloch, Tadeusz},
  issn         = {0306-4522},
  keyword      = {Brain ischemia,Calcium/calmodulin-dependent protein kinase II,Cell death,Phosphorylation,Tolerance},
  language     = {eng},
  number       = {4},
  pages        = {665--674},
  publisher    = {Elsevier},
  series       = {Neuroscience},
  title        = {Subcellular distribution and autophosphorylation of calcium/calmodulin- dependent protein kinase II-α in rat hippocampus in a model of ischemic tolerance},
  url          = {http://dx.doi.org/10.1016/S0306-4522(99)00586-2},
  volume       = {96},
  year         = {2000},
}