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Labeling and testing of (99m)Tc streptokinase for the diagnosis of deep vein thrombosis

Persson, B. R.R. LU orcid and Kempi, V. (1975) In Journal of Nuclear Medicine 16(6). p.474-477
Abstract

A detailed study has been made of the method for labeling streptokinase with 99Tc(m) in order to get a radioactive indicator for rapid scintigraphic visualization of thrombi and emboli. The best method found for preparing 99Tc(m) streptokinase was by reducing 99Tc(m) pertechnetate with 2 μmole SnCl2 at pH 0.7 and then adding 50,000 to 75,000 IU of streptokinase at a final pH of about 2. After 1 hr of equilibration, the labeling efficiency was 75 to 80% as determined by the method of gel chromatography column scanning. The dynamic behavior and distribution of 99Tc(m) streptokinase in different organs was studied in rabbits where a high uptake was found in liver. In a clinical... (More)

A detailed study has been made of the method for labeling streptokinase with 99Tc(m) in order to get a radioactive indicator for rapid scintigraphic visualization of thrombi and emboli. The best method found for preparing 99Tc(m) streptokinase was by reducing 99Tc(m) pertechnetate with 2 μmole SnCl2 at pH 0.7 and then adding 50,000 to 75,000 IU of streptokinase at a final pH of about 2. After 1 hr of equilibration, the labeling efficiency was 75 to 80% as determined by the method of gel chromatography column scanning. The dynamic behavior and distribution of 99Tc(m) streptokinase in different organs was studied in rabbits where a high uptake was found in liver. In a clinical investigation using 99Tc(m) streptokinase prepared according to this work, however, the liver uptake in man was only 10 to 20%.

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author
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publishing date
type
Contribution to journal
publication status
published
in
Journal of Nuclear Medicine
volume
16
issue
6
pages
4 pages
publisher
Society of Nuclear Medicine
external identifiers
  • scopus:0016591416
  • pmid:1159502
ISSN
0161-5505
language
English
LU publication?
yes
id
d9be062f-af6c-4637-b079-41d7a7b32435
date added to LUP
2020-08-30 21:06:53
date last changed
2024-01-02 17:29:38
@article{d9be062f-af6c-4637-b079-41d7a7b32435,
  abstract     = {{<p>A detailed study has been made of the method for labeling streptokinase with <sup>99</sup>Tc(m) in order to get a radioactive indicator for rapid scintigraphic visualization of thrombi and emboli. The best method found for preparing <sup>99</sup>Tc(m) streptokinase was by reducing <sup>99</sup>Tc(m) pertechnetate with 2 μmole SnCl<sub>2</sub> at pH 0.7 and then adding 50,000 to 75,000 IU of streptokinase at a final pH of about 2. After 1 hr of equilibration, the labeling efficiency was 75 to 80% as determined by the method of gel chromatography column scanning. The dynamic behavior and distribution of <sup>99</sup>Tc(m) streptokinase in different organs was studied in rabbits where a high uptake was found in liver. In a clinical investigation using <sup>99</sup>Tc(m) streptokinase prepared according to this work, however, the liver uptake in man was only 10 to 20%.</p>}},
  author       = {{Persson, B. R.R. and Kempi, V.}},
  issn         = {{0161-5505}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{6}},
  pages        = {{474--477}},
  publisher    = {{Society of Nuclear Medicine}},
  series       = {{Journal of Nuclear Medicine}},
  title        = {{Labeling and testing of (99m)Tc streptokinase for the diagnosis of deep vein thrombosis}},
  volume       = {{16}},
  year         = {{1975}},
}