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Synthesis of fluorescent molecularly imprinted nanoparticles for turn-on fluorescence assay using one-pot synthetic method and a preliminary microfluidic approach

Li, Qianjin LU ; Jiang, Lingdong LU ; Kamra, Tripta LU and Ye, Lei LU (2018) In Polymer (United Kingdom) 138. p.352-358
Abstract

Fluorescent molecularly imprinted polymers (MIPs) have received considerable attention for their promising applications in sensing, imaging and diagnostics. Different synthetic methods have been reported to prepare fluorescent MIPs. Here, one-pot polymerization method was developed to synthesize fluorescent MIP nanoparticles (NPs) bearing a suitable fluorescent label as the fluorescence acceptor. The MIP NPs can emit strong fluorescence based on fluorescence resonance energy transfer (FRET) when they bind to analytes acting as the fluorescence donor. The one-pot synthetic method is straightforward, less time-consuming and easy to optimize the loading of fluorescence acceptor. Additionally, a preliminary microfluidic reactor was designed... (More)

Fluorescent molecularly imprinted polymers (MIPs) have received considerable attention for their promising applications in sensing, imaging and diagnostics. Different synthetic methods have been reported to prepare fluorescent MIPs. Here, one-pot polymerization method was developed to synthesize fluorescent MIP nanoparticles (NPs) bearing a suitable fluorescent label as the fluorescence acceptor. The MIP NPs can emit strong fluorescence based on fluorescence resonance energy transfer (FRET) when they bind to analytes acting as the fluorescence donor. The one-pot synthetic method is straightforward, less time-consuming and easy to optimize the loading of fluorescence acceptor. Additionally, a preliminary microfluidic reactor was designed for preparing such fluorescent MIP NPs with more uniform size in a continuous process for the first time. The fluorescent MIP NPs can be used for selective detection of the analytical target in a FRET-based turn-on fluorescence assay which is easy to carry out with no tedious separation steps.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fluorescence resonance energy transfer, Molecularly imprinted polymer, One-pot synthesis
in
Polymer (United Kingdom)
volume
138
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85041476980
ISSN
0032-3861
DOI
10.1016/j.polymer.2018.01.086
language
English
LU publication?
yes
id
da0b2303-6bdc-422f-a504-e802c72556fa
date added to LUP
2018-02-20 13:44:26
date last changed
2018-06-10 05:27:00
@article{da0b2303-6bdc-422f-a504-e802c72556fa,
  abstract     = {<p>Fluorescent molecularly imprinted polymers (MIPs) have received considerable attention for their promising applications in sensing, imaging and diagnostics. Different synthetic methods have been reported to prepare fluorescent MIPs. Here, one-pot polymerization method was developed to synthesize fluorescent MIP nanoparticles (NPs) bearing a suitable fluorescent label as the fluorescence acceptor. The MIP NPs can emit strong fluorescence based on fluorescence resonance energy transfer (FRET) when they bind to analytes acting as the fluorescence donor. The one-pot synthetic method is straightforward, less time-consuming and easy to optimize the loading of fluorescence acceptor. Additionally, a preliminary microfluidic reactor was designed for preparing such fluorescent MIP NPs with more uniform size in a continuous process for the first time. The fluorescent MIP NPs can be used for selective detection of the analytical target in a FRET-based turn-on fluorescence assay which is easy to carry out with no tedious separation steps.</p>},
  author       = {Li, Qianjin and Jiang, Lingdong and Kamra, Tripta and Ye, Lei},
  issn         = {0032-3861},
  keyword      = {Fluorescence resonance energy transfer,Molecularly imprinted polymer,One-pot synthesis},
  language     = {eng},
  month        = {02},
  pages        = {352--358},
  publisher    = {Elsevier},
  series       = {Polymer (United Kingdom)},
  title        = {Synthesis of fluorescent molecularly imprinted nanoparticles for turn-on fluorescence assay using one-pot synthetic method and a preliminary microfluidic approach},
  url          = {http://dx.doi.org/10.1016/j.polymer.2018.01.086},
  volume       = {138},
  year         = {2018},
}