Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30
(2000) In Applied Biochemistry and Biotechnology 89(1). p.31-42- Abstract
- Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain... (More)
- Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain combinations. Two enzyme activities, cellulase and beta -glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was 1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity even at the highest concentration. However, beta -glucosidase activity was increased with increasing acetic acid concentration. Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and beta -glucosidase production. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911329
- author
- Szengyel, Z and Zacchi, Guido LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Trichoderma reesei RUT C30, cellulase, steam-pretreated willow, inhibition, acetic acid, furfural
- in
- Applied Biochemistry and Biotechnology
- volume
- 89
- issue
- 1
- pages
- 31 - 42
- publisher
- Humana Press
- external identifiers
-
- wos:000089949300003
- scopus:0033792370
- ISSN
- 1559-0291
- DOI
- 10.1385/ABAB:89:1:31
- language
- English
- LU publication?
- yes
- id
- da1578bb-ca12-46d7-a668-93307b507127 (old id 3911329)
- date added to LUP
- 2016-04-01 11:39:24
- date last changed
- 2023-12-09 17:37:55
@article{da1578bb-ca12-46d7-a668-93307b507127,
abstract = {{Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain combinations. Two enzyme activities, cellulase and beta -glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was 1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity even at the highest concentration. However, beta -glucosidase activity was increased with increasing acetic acid concentration. Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and beta -glucosidase production.}},
author = {{Szengyel, Z and Zacchi, Guido}},
issn = {{1559-0291}},
keywords = {{Trichoderma reesei RUT C30; cellulase; steam-pretreated willow; inhibition; acetic acid; furfural}},
language = {{eng}},
number = {{1}},
pages = {{31--42}},
publisher = {{Humana Press}},
series = {{Applied Biochemistry and Biotechnology}},
title = {{Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30}},
url = {{http://dx.doi.org/10.1385/ABAB:89:1:31}},
doi = {{10.1385/ABAB:89:1:31}},
volume = {{89}},
year = {{2000}},
}