Synthesizing a Hybrid Nanocomposite as an Affinity Adsorbent through Surface-Initiated Atom Transfer Radical Polymerization Catalyzed by Myoglobin
(2021) In ACS Omega 6(15). p.10462-10474- Abstract
A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic... (More)
A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic acid ligand was immobilized on the side chain of the grafted brushes during a series of postmodification reactions to create a boronate affinity adsorbent. The ability to selectively bind glycoproteins (ovalbumin and glycated hemoglobin) via boronic acid was assessed at two different temperatures (20 and 40 °C), where Si@pNIPA-b-APMABA (163 mg OVA/g of particle) displayed an approximately 1.5-fold higher capacity than Si@p(NIPA-co-APMA)BA (107 mg OVA/g of particle). In addition to selective binding to glycoproteins, the nanocomposites exhibited selective binding for myoglobin due to the molecular imprinting effect during the postmodification process, that is, 72 and 111 mg Mb/g for Si@p(NIPA-co-APMA)BA and Si@pNIPA-b-pAPMABA, respectively.
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- author
- Hajizadeh, Solmaz LU ; Bülow, Leif LU and Ye, Lei LU
- organization
- publishing date
- 2021-04-20
- type
- Contribution to journal
- publication status
- published
- subject
- in
- ACS Omega
- volume
- 6
- issue
- 15
- pages
- 13 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85105036681
- pmid:34056199
- ISSN
- 2470-1343
- DOI
- 10.1021/acsomega.1c00955
- language
- English
- LU publication?
- yes
- id
- da5ab983-9d3b-4aba-a7bf-07b04c911b70
- date added to LUP
- 2021-05-31 13:21:05
- date last changed
- 2024-08-24 18:44:00
@article{da5ab983-9d3b-4aba-a7bf-07b04c911b70, abstract = {{<p>A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic acid ligand was immobilized on the side chain of the grafted brushes during a series of postmodification reactions to create a boronate affinity adsorbent. The ability to selectively bind glycoproteins (ovalbumin and glycated hemoglobin) via boronic acid was assessed at two different temperatures (20 and 40 °C), where Si@pNIPA-b-APMABA (163 mg OVA/g of particle) displayed an approximately 1.5-fold higher capacity than Si@p(NIPA-co-APMA)BA (107 mg OVA/g of particle). In addition to selective binding to glycoproteins, the nanocomposites exhibited selective binding for myoglobin due to the molecular imprinting effect during the postmodification process, that is, 72 and 111 mg Mb/g for Si@p(NIPA-co-APMA)BA and Si@pNIPA-b-pAPMABA, respectively. </p>}}, author = {{Hajizadeh, Solmaz and Bülow, Leif and Ye, Lei}}, issn = {{2470-1343}}, language = {{eng}}, month = {{04}}, number = {{15}}, pages = {{10462--10474}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Omega}}, title = {{Synthesizing a Hybrid Nanocomposite as an Affinity Adsorbent through Surface-Initiated Atom Transfer Radical Polymerization Catalyzed by Myoglobin}}, url = {{http://dx.doi.org/10.1021/acsomega.1c00955}}, doi = {{10.1021/acsomega.1c00955}}, volume = {{6}}, year = {{2021}}, }