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Synthesizing a Hybrid Nanocomposite as an Affinity Adsorbent through Surface-Initiated Atom Transfer Radical Polymerization Catalyzed by Myoglobin

Hajizadeh, Solmaz LU orcid ; Bülow, Leif LU and Ye, Lei LU orcid (2021) In ACS Omega 6(15). p.10462-10474
Abstract

A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic... (More)

A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic acid ligand was immobilized on the side chain of the grafted brushes during a series of postmodification reactions to create a boronate affinity adsorbent. The ability to selectively bind glycoproteins (ovalbumin and glycated hemoglobin) via boronic acid was assessed at two different temperatures (20 and 40 °C), where Si@pNIPA-b-APMABA (163 mg OVA/g of particle) displayed an approximately 1.5-fold higher capacity than Si@p(NIPA-co-APMA)BA (107 mg OVA/g of particle). In addition to selective binding to glycoproteins, the nanocomposites exhibited selective binding for myoglobin due to the molecular imprinting effect during the postmodification process, that is, 72 and 111 mg Mb/g for Si@p(NIPA-co-APMA)BA and Si@pNIPA-b-pAPMABA, respectively.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Omega
volume
6
issue
15
pages
13 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85105036681
  • pmid:34056199
ISSN
2470-1343
DOI
10.1021/acsomega.1c00955
language
English
LU publication?
yes
id
da5ab983-9d3b-4aba-a7bf-07b04c911b70
date added to LUP
2021-05-31 13:21:05
date last changed
2025-10-14 12:57:02
@article{da5ab983-9d3b-4aba-a7bf-07b04c911b70,
  abstract     = {{<p>A hybrid bifunctional core-shell nanostructure was synthesized for the first time via surface-initiated atom transfer radical polymerization (SI-ATRP) using myoglobin as a biocatalyst (ATRPase) in an aqueous solution. N-Isopropyl acrylamide (NIPA) and N-(3-aminopropyl)methacrylamide (APMA) were applied to graft flexible polymer brushes onto initiator-functionalized silica nanoparticles. Two different approaches were implemented to form the core-shell nanocomposite: (a) random copolymerization, Si@p(NIPA-co-APMA) and (b) sequential block copolymerization, Si@pNIPA-b-pAPMA. These nanocomposites can be used as versatile intermediates, thereby leading to different types of materials for targeted applications. In this work, a phenylboronic acid ligand was immobilized on the side chain of the grafted brushes during a series of postmodification reactions to create a boronate affinity adsorbent. The ability to selectively bind glycoproteins (ovalbumin and glycated hemoglobin) via boronic acid was assessed at two different temperatures (20 and 40 °C), where Si@pNIPA-b-APMABA (163 mg OVA/g of particle) displayed an approximately 1.5-fold higher capacity than Si@p(NIPA-co-APMA)BA (107 mg OVA/g of particle). In addition to selective binding to glycoproteins, the nanocomposites exhibited selective binding for myoglobin due to the molecular imprinting effect during the postmodification process, that is, 72 and 111 mg Mb/g for Si@p(NIPA-co-APMA)BA and Si@pNIPA-b-pAPMABA, respectively. </p>}},
  author       = {{Hajizadeh, Solmaz and Bülow, Leif and Ye, Lei}},
  issn         = {{2470-1343}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{15}},
  pages        = {{10462--10474}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Omega}},
  title        = {{Synthesizing a Hybrid Nanocomposite as an Affinity Adsorbent through Surface-Initiated Atom Transfer Radical Polymerization Catalyzed by Myoglobin}},
  url          = {{http://dx.doi.org/10.1021/acsomega.1c00955}},
  doi          = {{10.1021/acsomega.1c00955}},
  volume       = {{6}},
  year         = {{2021}},
}