Environmentally induced DNA methylation is inherited across generations in an aquatic keystone species
(2022) In iScience 25(5).- Abstract
- Transgenerational inheritance of environmentally induced epigenetic marks can have significant impacts on eco-evolutionary dynamics, but the phenomenon remains controversial in ecological model systems. We used whole-genome bisulfite sequencing of individual water fleas (Daphnia magna) to assess whether environmentally induced DNA methylation is transgenerationally inherited. Genetically identical females were exposed to one of three natural stressors, or a de-methylating drug, and their offspring were propagated clonally for four generations under control conditions. We identified between 70 and 225 differentially methylated CpG positions (DMPs) in F1 individuals whose mothers were exposed to a natural stressor. Roughly half of these... (More)
- Transgenerational inheritance of environmentally induced epigenetic marks can have significant impacts on eco-evolutionary dynamics, but the phenomenon remains controversial in ecological model systems. We used whole-genome bisulfite sequencing of individual water fleas (Daphnia magna) to assess whether environmentally induced DNA methylation is transgenerationally inherited. Genetically identical females were exposed to one of three natural stressors, or a de-methylating drug, and their offspring were propagated clonally for four generations under control conditions. We identified between 70 and 225 differentially methylated CpG positions (DMPs) in F1 individuals whose mothers were exposed to a natural stressor. Roughly half of these environmentally induced DMPs persisted until generation F4. In contrast, treatment with the drug demonstrated that pervasive hypomethylation upon exposure is reset almost completely after one generation. These results suggest that environmentally induced DNA methylation is non-random and stably inherited across generations in Daphnia, making epigenetic inheritance a putative factor in the eco-evolutionary dynamics of freshwater communities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/da9e326d-8d42-46d3-811a-ebe95f14deaa
- author
- Feiner, Nathalie
LU
; Radersma, Reinder
LU
; Vasquez, Louella
LU
; Ringnér, Markus
LU
; Nystedt, Björn ; Raine, Amanda ; Tobi, Elmar W. ; Heijmans, Bastiaan T. and Uller, Tobias LU
- organization
- publishing date
- 2022-05-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Molecular genetics, DNA methylation, Epigenetics
- in
- iScience
- volume
- 25
- issue
- 5
- article number
- 104303
- pages
- 18 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85129924515
- pmid:35573201
- ISSN
- 2589-0042
- DOI
- 10.1016/j.isci.2022.104303
- language
- English
- LU publication?
- yes
- id
- da9e326d-8d42-46d3-811a-ebe95f14deaa
- date added to LUP
- 2022-05-09 15:41:40
- date last changed
- 2022-08-09 03:00:09
@article{da9e326d-8d42-46d3-811a-ebe95f14deaa, abstract = {{Transgenerational inheritance of environmentally induced epigenetic marks can have significant impacts on eco-evolutionary dynamics, but the phenomenon remains controversial in ecological model systems. We used whole-genome bisulfite sequencing of individual water fleas (Daphnia magna) to assess whether environmentally induced DNA methylation is transgenerationally inherited. Genetically identical females were exposed to one of three natural stressors, or a de-methylating drug, and their offspring were propagated clonally for four generations under control conditions. We identified between 70 and 225 differentially methylated CpG positions (DMPs) in F1 individuals whose mothers were exposed to a natural stressor. Roughly half of these environmentally induced DMPs persisted until generation F4. In contrast, treatment with the drug demonstrated that pervasive hypomethylation upon exposure is reset almost completely after one generation. These results suggest that environmentally induced DNA methylation is non-random and stably inherited across generations in Daphnia, making epigenetic inheritance a putative factor in the eco-evolutionary dynamics of freshwater communities.}}, author = {{Feiner, Nathalie and Radersma, Reinder and Vasquez, Louella and Ringnér, Markus and Nystedt, Björn and Raine, Amanda and Tobi, Elmar W. and Heijmans, Bastiaan T. and Uller, Tobias}}, issn = {{2589-0042}}, keywords = {{Molecular genetics; DNA methylation; Epigenetics}}, language = {{eng}}, month = {{05}}, number = {{5}}, publisher = {{Elsevier}}, series = {{iScience}}, title = {{Environmentally induced DNA methylation is inherited across generations in an aquatic keystone species}}, url = {{http://dx.doi.org/10.1016/j.isci.2022.104303}}, doi = {{10.1016/j.isci.2022.104303}}, volume = {{25}}, year = {{2022}}, }