Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody
Surugiu, Ioana ; Danielsson, B. LU ; Ye, L. LU
; Mosbach, K.
LU
and Haupt, K.
LU
(2001)
In Analytical Chemistry
73(3).
p.487-491
- Abstract
An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput... (More)
An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.
(Less)
- author
- Surugiu, Ioana
; Danielsson, B.
LU
; Ye, L.
LU
; Mosbach, K.
LU
and Haupt, K.
LU
- organization
- publishing date
- 2001-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 73
- issue
- 3
- pages
- 5 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:0035253704
- pmid:11217751
- ISSN
- 0003-2700
- DOI
- 10.1021/ac0011540
- language
- English
- LU publication?
- yes
- id
- dae65872-130e-49f1-84fc-2e7a17f31c8f
- date added to LUP
- 2019-06-18 09:01:57
- date last changed
- 2024-05-29 15:10:25
@article{dae65872-130e-49f1-84fc-2e7a17f31c8f,
abstract = {{<p>An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.</p>}},
author = {{Surugiu, Ioana and Danielsson, B. and Ye, L. and Mosbach, K. and Haupt, K.}},
issn = {{0003-2700}},
language = {{eng}},
month = {{02}},
number = {{3}},
pages = {{487--491}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody}},
url = {{http://dx.doi.org/10.1021/ac0011540}},
doi = {{10.1021/ac0011540}},
volume = {{73}},
year = {{2001}},
}