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Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody

Surugiu, Ioana ; Danielsson, B. LU ; Ye, L. LU ; Mosbach, K. LU and Haupt, K. LU (2001) In Analytical Chemistry 73(3). p.487-491
Abstract

An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput... (More)

An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
73
issue
3
pages
5 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:11217751
  • scopus:0035253704
ISSN
0003-2700
DOI
10.1021/ac0011540
language
English
LU publication?
yes
id
dae65872-130e-49f1-84fc-2e7a17f31c8f
date added to LUP
2019-06-18 09:01:57
date last changed
2021-04-06 01:55:22
@article{dae65872-130e-49f1-84fc-2e7a17f31c8f,
  abstract     = {<p>An imaging assay analogous to competitive enzyme immunoassays has been developed using a molecularly imprinted polymer instead of an antibody. The antigen 2,4-dichlorophenoxyacetic acid (2,4-D) was labeled with tobacco peroxidase, and the chemiluminescence reaction of luminol was used for detection. Microtiter plates (96 or 384 wells) were coated with polymer microspheres imprinted with 2,4-D, which were fixed in place by using poly(vinyl alcohol) as glue. In a competitive mode, the analyte-peroxidase conjugate was incubated with the free analyte in the microtiter plate, after which the bound fraction of the conjugate was quantified. After addition of the chemiluminescent substrates, light emission was measured in a high-throughput imaging format with a CCD camera. Calibration curves corresponding to analyte concentrations ranging from 0.01 to 100 μg/mL were obtained.</p>},
  author       = {Surugiu, Ioana and Danielsson, B. and Ye, L. and Mosbach, K. and Haupt, K.},
  issn         = {0003-2700},
  language     = {eng},
  month        = {02},
  number       = {3},
  pages        = {487--491},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Analytical Chemistry},
  title        = {Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody},
  url          = {http://dx.doi.org/10.1021/ac0011540},
  doi          = {10.1021/ac0011540},
  volume       = {73},
  year         = {2001},
}