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Variations in plasmid content during Escherichia coli cultivations detected by on-line flow injection processing

Nandakumar, M. P. ; Nordberg Karlsson, E. LU and Mattiasson, B. LU (2001) In Biotechnology Letters 23(14). p.1135-1140
Abstract

An integrated flow injection process for analysis of intracellular components of microbes has been used to monitor plasmid content in Escherichia coli cultivations inoculated with cells subcultured in the presence or absence of ampicillin. The system allows sampling, sample handling, cell disruption, separation of intracellular components, and analysis in a semi-on-line mode of operation. The time scale for the assay is in the range 15 min (plasmid peak) to 25 min (complete assay cycle). As expected, lower initial plasmid content was found using an inoculum subcultured in the absence of ampicillin. More importantly, significant decrease in plasmid content was detected in the later stages of the cultivations (grown in ampicillin... (More)

An integrated flow injection process for analysis of intracellular components of microbes has been used to monitor plasmid content in Escherichia coli cultivations inoculated with cells subcultured in the presence or absence of ampicillin. The system allows sampling, sample handling, cell disruption, separation of intracellular components, and analysis in a semi-on-line mode of operation. The time scale for the assay is in the range 15 min (plasmid peak) to 25 min (complete assay cycle). As expected, lower initial plasmid content was found using an inoculum subcultured in the absence of ampicillin. More importantly, significant decrease in plasmid content was detected in the later stages of the cultivations (grown in ampicillin containing medium) even when using inoculum subcultured in the presence of ampicillin. This illustrates the versatility of the system, which allows monitoring of plasmid content as the cultivation proceeds.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Flow injection analysis, Plasmid content
in
Biotechnology Letters
volume
23
issue
14
pages
6 pages
publisher
Springer
external identifiers
  • scopus:0034868026
ISSN
0141-5492
DOI
10.1023/A:1010568019191
language
English
LU publication?
yes
id
dae76a20-8a75-49df-b117-10c30c364345
date added to LUP
2018-11-14 21:05:21
date last changed
2020-02-12 09:44:28
@article{dae76a20-8a75-49df-b117-10c30c364345,
  abstract     = {<p>An integrated flow injection process for analysis of intracellular components of microbes has been used to monitor plasmid content in Escherichia coli cultivations inoculated with cells subcultured in the presence or absence of ampicillin. The system allows sampling, sample handling, cell disruption, separation of intracellular components, and analysis in a semi-on-line mode of operation. The time scale for the assay is in the range 15 min (plasmid peak) to 25 min (complete assay cycle). As expected, lower initial plasmid content was found using an inoculum subcultured in the absence of ampicillin. More importantly, significant decrease in plasmid content was detected in the later stages of the cultivations (grown in ampicillin containing medium) even when using inoculum subcultured in the presence of ampicillin. This illustrates the versatility of the system, which allows monitoring of plasmid content as the cultivation proceeds.</p>},
  author       = {Nandakumar, M. P. and Nordberg Karlsson, E. and Mattiasson, B.},
  issn         = {0141-5492},
  language     = {eng},
  month        = {09},
  number       = {14},
  pages        = {1135--1140},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Variations in plasmid content during Escherichia coli cultivations detected by on-line flow injection processing},
  url          = {http://dx.doi.org/10.1023/A:1010568019191},
  doi          = {10.1023/A:1010568019191},
  volume       = {23},
  year         = {2001},
}