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Backbone resonance assignment of Staphylococcal Enterotoxin H

Saline, Maria; Orekhov, Vladislav Yu.; Lindkvist-Petersson, Karin LU and Karlsson, B. G. (2010) In Biomolecular NMR Assignments 4(1). p.1-4
Abstract

The staphylococcal enterotoxin H (SEH; 217 aa, 25 kD) belongs to a family of superantigens that cause a massive immune response upon simultaneous binding to the T cell receptor (TCR) and the major histocompatibility complex class II. The SEH-TCR interaction is weak and amenable to studies using NMR methodology. Essentially, 2 mg of U{2H, 13C,15N}-labeled SEH was used for the complete sequential backbone assignment of SEH at 900 MHz. The protein secondary structure inferred from the chemical shift index (C α and Cβ) is in very good agreement with the secondary structure elements of the X-ray structure.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Assignment, MHC(II), Staphylococcal enterotoxin, Superantigen, T-cell receptor
in
Biomolecular NMR Assignments
volume
4
issue
1
pages
1 - 4
publisher
Springer
external identifiers
  • scopus:77951880390
ISSN
1874-2718
DOI
10.1007/s12104-009-9193-1
language
English
LU publication?
no
id
db149822-21bb-4476-bd8e-1cf1808f6a87
date added to LUP
2017-02-14 16:32:07
date last changed
2018-05-29 11:32:47
@article{db149822-21bb-4476-bd8e-1cf1808f6a87,
  abstract     = {<p>The staphylococcal enterotoxin H (SEH; 217 aa, 25 kD) belongs to a family of superantigens that cause a massive immune response upon simultaneous binding to the T cell receptor (TCR) and the major histocompatibility complex class II. The SEH-TCR interaction is weak and amenable to studies using NMR methodology. Essentially, 2 mg of U{<sup>2</sup>H, <sup>13</sup>C,<sup>15</sup>N}-labeled SEH was used for the complete sequential backbone assignment of SEH at 900 MHz. The protein secondary structure inferred from the chemical shift index (C <sub>α</sub> and C<sub>β</sub>) is in very good agreement with the secondary structure elements of the X-ray structure.</p>},
  author       = {Saline, Maria and Orekhov, Vladislav Yu. and Lindkvist-Petersson, Karin and Karlsson, B. G.},
  issn         = {1874-2718},
  keyword      = {Assignment,MHC(II),Staphylococcal enterotoxin,Superantigen,T-cell receptor},
  language     = {eng},
  number       = {1},
  pages        = {1--4},
  publisher    = {Springer},
  series       = {Biomolecular NMR Assignments},
  title        = {Backbone resonance assignment of Staphylococcal Enterotoxin H},
  url          = {http://dx.doi.org/10.1007/s12104-009-9193-1},
  volume       = {4},
  year         = {2010},
}