Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems

Efremenko, Elena N. ; Lyagin, Ilya V. ; Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU (2009) In Applied Biochemistry and Biotechnology 159(1). p.251-260
Abstract
New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immobilized biocatalyst, Dried/reswollen, Organophosphorus hydrolase, Polyhistidine sequence, Organophosphorous compound
in
Applied Biochemistry and Biotechnology
volume
159
issue
1
pages
251 - 260
publisher
Humana Press
external identifiers
  • wos:000269515600023
  • scopus:70350568768
  • pmid:19551352
ISSN
1559-0291
DOI
10.1007/s12010-009-8564-5
language
English
LU publication?
yes
id
db8593cf-3a0b-49e3-830e-55ec1b621787 (old id 1476816)
date added to LUP
2016-04-01 12:05:02
date last changed
2025-10-14 10:18:28
@article{db8593cf-3a0b-49e3-830e-55ec1b621787,
  abstract     = {{New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.}},
  author       = {{Efremenko, Elena N. and Lyagin, Ilya V. and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo}},
  issn         = {{1559-0291}},
  keywords     = {{immobilized biocatalyst; Dried/reswollen; Organophosphorus hydrolase; Polyhistidine sequence; Organophosphorous compound}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{251--260}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems}},
  url          = {{http://dx.doi.org/10.1007/s12010-009-8564-5}},
  doi          = {{10.1007/s12010-009-8564-5}},
  volume       = {{159}},
  year         = {{2009}},
}