Characterisation of the microbial community in indoor environments: Critical evaluation of sample storage conditions
(2005) 10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005) p.2421-2424- Abstract
- Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be... (More)
- Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be used for characterisation of microbial communities in indoor environments as regards fungal biomass and endotoxin as well as relative amounts of Gram-negative/Gram-positive bacteria. The present investigation aims to show the influence of different storage conditions on stability of the markers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1406424
- author
- Sebastian, Aleksandra LU and Larsson, L
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- gas chromatography-mass spectrometry, microbial markers, house dust
- host publication
- Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5
- pages
- 2421 - 2424
- publisher
- Tsinghua University Press
- conference name
- 10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
- conference location
- Beijing, China
- conference dates
- 2005-09-04 - 2005-09-09
- external identifiers
-
- wos:000233831203089
- language
- English
- LU publication?
- yes
- id
- dbb26f33-1475-408f-b6b2-47e0062f907b (old id 1406424)
- date added to LUP
- 2016-04-04 10:47:09
- date last changed
- 2020-06-15 07:51:36
@inproceedings{dbb26f33-1475-408f-b6b2-47e0062f907b,
abstract = {{Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be used for characterisation of microbial communities in indoor environments as regards fungal biomass and endotoxin as well as relative amounts of Gram-negative/Gram-positive bacteria. The present investigation aims to show the influence of different storage conditions on stability of the markers.}},
author = {{Sebastian, Aleksandra and Larsson, L}},
booktitle = {{Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5}},
keywords = {{gas chromatography-mass spectrometry; microbial markers; house dust}},
language = {{eng}},
pages = {{2421--2424}},
publisher = {{Tsinghua University Press}},
title = {{Characterisation of the microbial community in indoor environments: Critical evaluation of sample storage conditions}},
year = {{2005}},
}