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Tyrosine Phosphorylation and Activation of Mitogen‐ Activated Protein Kinase in the Rat Brain Following Transient Cerebral Ischemia

Hu, Bing‐Ren and Wieloch, Tadeusz LU (1994) In Journal of Neurochemistry 62(4). p.1357-1367
Abstract

Abstract: Activation of trophic factor receptors stimulates tyrosine phosphorylation on proteins and supports neuronal survival. We report that in the recovery phase following reversible cerebral ischemia, tyrosine phosphorylation increases in the membrane fraction of the resistant hippocampal CA3/dentate gyrus (DG) region, whereas in the sensitive CA1 region or striatum, tyrosine phosphorylation is less marked or decreases. In the cytosolic fractions, a 42‐kDa protein, identified as mitogen‐activated protein (MAP) kinase, is markedly phosphorylated and activated immediately following ischemia, in particular in CA3/DG, but not in striatum. In the CA1 region, phosphorylation of MAP kinase is less intense and decreases later during re‐... (More)

Abstract: Activation of trophic factor receptors stimulates tyrosine phosphorylation on proteins and supports neuronal survival. We report that in the recovery phase following reversible cerebral ischemia, tyrosine phosphorylation increases in the membrane fraction of the resistant hippocampal CA3/dentate gyrus (DG) region, whereas in the sensitive CA1 region or striatum, tyrosine phosphorylation is less marked or decreases. In the cytosolic fractions, a 42‐kDa protein, identified as mitogen‐activated protein (MAP) kinase, is markedly phosphorylated and activated immediately following ischemia, in particular in CA3/DG, but not in striatum. In the CA1 region, phosphorylation of MAP kinase is less intense and decreases later during re‐ perfusion, which could explain the delay of neuronal degeneration in this structure. The data suggest that in ischemia‐resistant neurons the growth factor receptor‐coupled signaling cascade is stimulated and, through its effects on DNA transcription and mRNA translation, supports neuronal survival.

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author
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Glutamate, Hippocampus, Ischemia, Mitogen‐activated kinase, Neuronal death, Trophic factors, Tyrosine phosphorylation
in
Journal of Neurochemistry
volume
62
issue
4
pages
11 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:7510779
  • scopus:0028353865
ISSN
0022-3042
DOI
10.1046/j.1471-4159.1994.62041357.x
language
English
LU publication?
yes
id
dbb53052-bd66-45a9-8306-25d7c2ba0ffd
date added to LUP
2019-06-13 16:14:23
date last changed
2024-01-01 10:13:52
@article{dbb53052-bd66-45a9-8306-25d7c2ba0ffd,
  abstract     = {{<p>Abstract:  Activation of trophic factor receptors stimulates tyrosine phosphorylation on proteins and supports neuronal survival. We report that in the recovery phase following reversible cerebral ischemia, tyrosine phosphorylation increases in the membrane fraction of the resistant hippocampal CA3/dentate gyrus (DG) region, whereas in the sensitive CA1 region or striatum, tyrosine phosphorylation is less marked or decreases. In the cytosolic fractions, a 42‐kDa protein, identified as mitogen‐activated protein (MAP) kinase, is markedly phosphorylated and activated immediately following ischemia, in particular in CA3/DG, but not in striatum. In the CA1 region, phosphorylation of MAP kinase is less intense and decreases later during re‐ perfusion, which could explain the delay of neuronal degeneration in this structure. The data suggest that in ischemia‐resistant neurons the growth factor receptor‐coupled signaling cascade is stimulated and, through its effects on DNA transcription and mRNA translation, supports neuronal survival.</p>}},
  author       = {{Hu, Bing‐Ren and Wieloch, Tadeusz}},
  issn         = {{0022-3042}},
  keywords     = {{Glutamate; Hippocampus; Ischemia; Mitogen‐activated kinase; Neuronal death; Trophic factors; Tyrosine phosphorylation}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{4}},
  pages        = {{1357--1367}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Neurochemistry}},
  title        = {{Tyrosine Phosphorylation and Activation of Mitogen‐ Activated Protein Kinase in the Rat Brain Following Transient Cerebral Ischemia}},
  url          = {{http://dx.doi.org/10.1046/j.1471-4159.1994.62041357.x}},
  doi          = {{10.1046/j.1471-4159.1994.62041357.x}},
  volume       = {{62}},
  year         = {{1994}},
}