Fast determination of antibiotics in whole blood
(2013) In Clinical Microbiology and Infection 19(9). p.869-874- Abstract
- There is a need for analytical methods capable of monitoring blood antibiotic levels in real time. Here we present a method for quantifying antibiotic levels in whole blood that does not require any sample pretreatment. The tests employ the enzyme penicillinase to assay for penicillin G, penicillin V and ampicillin using a flow-injected biosensor, the Enzyme Thermistor. Optimal flow rates, sample volumes and pH were determined to be 0.5mL/min, 100L and 7.0, respectively. Analysis of the antibiotics diluted in buffer gave a linear range of 0.17-5.0mM. Calibration curves prepared using blood spiked with the antibiotics gave a linear range of 0.17-2.0mM. Linear regression values for all of the calibration curves were 0.998 or higher. Assay... (More)
- There is a need for analytical methods capable of monitoring blood antibiotic levels in real time. Here we present a method for quantifying antibiotic levels in whole blood that does not require any sample pretreatment. The tests employ the enzyme penicillinase to assay for penicillin G, penicillin V and ampicillin using a flow-injected biosensor, the Enzyme Thermistor. Optimal flow rates, sample volumes and pH were determined to be 0.5mL/min, 100L and 7.0, respectively. Analysis of the antibiotics diluted in buffer gave a linear range of 0.17-5.0mM. Calibration curves prepared using blood spiked with the antibiotics gave a linear range of 0.17-2.0mM. Linear regression values for all of the calibration curves were 0.998 or higher. Assay cycle time was 5min. The relative standard deviation value for 100 determinations of a mock blood sample spiked with penicillin G was 6.71%. Despite the elimination of sample pretreatment, no detectable clogging or signal drift was observed. The assay provides a fast, simple, reliable analytical method for determining antibiotic concentrations in blood without the need for any sample pretreatment. This is an important first step towards developing a device capable of real-time monitoring of antibiotic levels in whole blood. The technology has the potential to significantly improve the outcomes of patients undergoing critical care. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4027139
- author
- Chen, Q. ; Andersson, A. ; Mecklenburg, M. and Xie, Bin LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antibiotics, biosensor, blood, critical care, penicillinase
- in
- Clinical Microbiology and Infection
- volume
- 19
- issue
- 9
- pages
- 869 - 874
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000323201300026
- scopus:84882588992
- pmid:23190049
- ISSN
- 1469-0691
- DOI
- 10.1111/1469-0691.12074
- language
- English
- LU publication?
- yes
- id
- dc144279-b98e-46a9-bc86-8af51b54d008 (old id 4027139)
- date added to LUP
- 2016-04-01 10:04:59
- date last changed
- 2022-01-25 19:28:54
@article{dc144279-b98e-46a9-bc86-8af51b54d008,
abstract = {{There is a need for analytical methods capable of monitoring blood antibiotic levels in real time. Here we present a method for quantifying antibiotic levels in whole blood that does not require any sample pretreatment. The tests employ the enzyme penicillinase to assay for penicillin G, penicillin V and ampicillin using a flow-injected biosensor, the Enzyme Thermistor. Optimal flow rates, sample volumes and pH were determined to be 0.5mL/min, 100L and 7.0, respectively. Analysis of the antibiotics diluted in buffer gave a linear range of 0.17-5.0mM. Calibration curves prepared using blood spiked with the antibiotics gave a linear range of 0.17-2.0mM. Linear regression values for all of the calibration curves were 0.998 or higher. Assay cycle time was 5min. The relative standard deviation value for 100 determinations of a mock blood sample spiked with penicillin G was 6.71%. Despite the elimination of sample pretreatment, no detectable clogging or signal drift was observed. The assay provides a fast, simple, reliable analytical method for determining antibiotic concentrations in blood without the need for any sample pretreatment. This is an important first step towards developing a device capable of real-time monitoring of antibiotic levels in whole blood. The technology has the potential to significantly improve the outcomes of patients undergoing critical care.}},
author = {{Chen, Q. and Andersson, A. and Mecklenburg, M. and Xie, Bin}},
issn = {{1469-0691}},
keywords = {{Antibiotics; biosensor; blood; critical care; penicillinase}},
language = {{eng}},
number = {{9}},
pages = {{869--874}},
publisher = {{Wiley-Blackwell}},
series = {{Clinical Microbiology and Infection}},
title = {{Fast determination of antibiotics in whole blood}},
url = {{http://dx.doi.org/10.1111/1469-0691.12074}},
doi = {{10.1111/1469-0691.12074}},
volume = {{19}},
year = {{2013}},
}