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Junctional Adhesion Molecule 3 Expression in the Mouse Airway Epithelium Is Linked to Multiciliated Cells

Mateos-Quiros, Clara Maria ; Garrido-Jimenez, Sergio ; Álvarez-Hernán, Guadalupe LU ; Diaz-Chamorro, Selene ; Barrera-Lopez, Juan Francisco ; Francisco-Morcillo, Javier ; Roman, Angel Carlos ; Centeno, Francisco and Carvajal-Gonzalez, Jose Maria (2021) In Frontiers in Cell and Developmental Biology 9.
Abstract

Tight-junction (TJ) proteins are essential for establishing the barrier function between neighbor epithelial cells, but also for recognition of pathogens or cell migration. Establishing the expression pattern and localization of different TJ proteins will help to understand the development and physiology of the airway. Here we identify that the junctional adhesion molecule 3 (Jam3) expression is restricted to multiciliated cells (MCCs) in the airway epithelium. In vitro, Jam3 expression varies along airway basal stem cell (BSC) differentiation and upon DAPT treatment or IL6 exposure. However, Jam3 is not required for BSC differentiation to specific cell types. In addition, we found that MCC lacking Jam3 display normal cilia morphology... (More)

Tight-junction (TJ) proteins are essential for establishing the barrier function between neighbor epithelial cells, but also for recognition of pathogens or cell migration. Establishing the expression pattern and localization of different TJ proteins will help to understand the development and physiology of the airway. Here we identify that the junctional adhesion molecule 3 (Jam3) expression is restricted to multiciliated cells (MCCs) in the airway epithelium. In vitro, Jam3 expression varies along airway basal stem cell (BSC) differentiation and upon DAPT treatment or IL6 exposure. However, Jam3 is not required for BSC differentiation to specific cell types. In addition, we found that MCC lacking Jam3 display normal cilia morphology and cilia beating frequency with a delay in BB assembly/positioning in MCCs during differentiation. Remarkably, Jam3 in MCC is mostly localized to subapical organelles, which are negative for the apical recycling endosome marker Rab11 and positive for EEA1. Our data show that Jam3 expression is connected to mature MCC in the airway epithelium and suggest a Jam3 role unrelated to its known barrier function.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Cell and Developmental Biology
volume
9
article number
622515
publisher
Frontiers Media S. A.
external identifiers
  • pmid:34395412
  • scopus:85112291364
ISSN
2296-634X
DOI
10.3389/fcell.2021.622515
language
English
LU publication?
no
additional info
Copyright © 2021 Mateos-Quiros, Garrido-Jimenez, Álvarez-Hernán, Diaz-Chamorro, Barrera-Lopez, Francisco-Morcillo, Roman, Centeno and Carvajal-Gonzalez.
id
dc28448f-cfd3-41d0-b68f-5f96e476c072
date added to LUP
2021-12-15 14:02:51
date last changed
2024-06-01 22:05:29
@article{dc28448f-cfd3-41d0-b68f-5f96e476c072,
  abstract     = {{<p>Tight-junction (TJ) proteins are essential for establishing the barrier function between neighbor epithelial cells, but also for recognition of pathogens or cell migration. Establishing the expression pattern and localization of different TJ proteins will help to understand the development and physiology of the airway. Here we identify that the junctional adhesion molecule 3 (Jam3) expression is restricted to multiciliated cells (MCCs) in the airway epithelium. In vitro, Jam3 expression varies along airway basal stem cell (BSC) differentiation and upon DAPT treatment or IL6 exposure. However, Jam3 is not required for BSC differentiation to specific cell types. In addition, we found that MCC lacking Jam3 display normal cilia morphology and cilia beating frequency with a delay in BB assembly/positioning in MCCs during differentiation. Remarkably, Jam3 in MCC is mostly localized to subapical organelles, which are negative for the apical recycling endosome marker Rab11 and positive for EEA1. Our data show that Jam3 expression is connected to mature MCC in the airway epithelium and suggest a Jam3 role unrelated to its known barrier function.</p>}},
  author       = {{Mateos-Quiros, Clara Maria and Garrido-Jimenez, Sergio and Álvarez-Hernán, Guadalupe and Diaz-Chamorro, Selene and Barrera-Lopez, Juan Francisco and Francisco-Morcillo, Javier and Roman, Angel Carlos and Centeno, Francisco and Carvajal-Gonzalez, Jose Maria}},
  issn         = {{2296-634X}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Cell and Developmental Biology}},
  title        = {{Junctional Adhesion Molecule 3 Expression in the Mouse Airway Epithelium Is Linked to Multiciliated Cells}},
  url          = {{http://dx.doi.org/10.3389/fcell.2021.622515}},
  doi          = {{10.3389/fcell.2021.622515}},
  volume       = {{9}},
  year         = {{2021}},
}