Dynamic perifusion to maintain and assess isolated pancreatic islets.
Sweet, I R ; Cook, D L ; Wiseman, R W ; Greenbaum, C J ; Lernmark, Åke LU
; Matsumoto, S
; Teague, J C
and Krohn, K A
(2002)
In Diabetes Technology & Therapeutics
4(1).
p.67-76
- Abstract
- Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells... (More)
- Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1124810
- author
- Sweet, I R
; Cook, D L
; Wiseman, R W
; Greenbaum, C J
; Lernmark, Åke
LU
; Matsumoto, S
; Teague, J C
and Krohn, K A
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diabetes Technology & Therapeutics
- volume
- 4
- issue
- 1
- pages
- 67 - 76
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- scopus:0036232564
- ISSN
- 1520-9156
- DOI
- 10.1089/15209150252924111
- language
- English
- LU publication?
- no
- id
- dc293cca-bec1-4e60-9a94-3c56d82b7e3a (old id 1124810)
- date added to LUP
- 2016-04-01 12:30:59
- date last changed
- 2022-01-27 06:10:50
@article{dc293cca-bec1-4e60-9a94-3c56d82b7e3a,
abstract = {{Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a}},
author = {{Sweet, I R and Cook, D L and Wiseman, R W and Greenbaum, C J and Lernmark, Åke and Matsumoto, S and Teague, J C and Krohn, K A}},
issn = {{1520-9156}},
language = {{eng}},
number = {{1}},
pages = {{67--76}},
publisher = {{Mary Ann Liebert, Inc.}},
series = {{Diabetes Technology & Therapeutics}},
title = {{Dynamic perifusion to maintain and assess isolated pancreatic islets.}},
url = {{http://dx.doi.org/10.1089/15209150252924111}},
doi = {{10.1089/15209150252924111}},
volume = {{4}},
year = {{2002}},
}