Magnetic resonance and diffusion tensor imaging of the adolescent rabbit growth plate of the knee
(2023) In Magnetic Resonance in Medicine 89(1). p.331-342- Abstract
Purpose: To assess the ability of MRI-DTI to evaluate growth plate morphology and activity compared with that of histomorphometry and micro-CT in rabbits. Methods: The hind limbs of female rabbits aged 16, 20, and 24 wk (n = 4 per age group) were studied using a 9.4T MRI scanner with a multi-gradient echo 3D sequence and DTI in 14 directions (b-value = 984 s/mm2). After MRI, the right and left hind limb were processed for histological analysis and micro-CT, respectively. The Wilcoxon signed-rank test was used to evaluate the height and volume of the growth plate. Intraclass correlation and Pearson correlation coefficient were used to evaluate the association between DTI metrics and age. Results: The growth plate height and... (More)
Purpose: To assess the ability of MRI-DTI to evaluate growth plate morphology and activity compared with that of histomorphometry and micro-CT in rabbits. Methods: The hind limbs of female rabbits aged 16, 20, and 24 wk (n = 4 per age group) were studied using a 9.4T MRI scanner with a multi-gradient echo 3D sequence and DTI in 14 directions (b-value = 984 s/mm2). After MRI, the right and left hind limb were processed for histological analysis and micro-CT, respectively. The Wilcoxon signed-rank test was used to evaluate the height and volume of the growth plate. Intraclass correlation and Pearson correlation coefficient were used to evaluate the association between DTI metrics and age. Results: The growth plate height and volume were similar for all modalities at each time point and age. Age was correlated with all tractography and DTI metrics in both the femur and tibia. A correlation was also observed between all the metrics at both sites. Tract number and volume declined with age; however, tract length did not show any changes. The fractional anisotropy color map showed lateral diffusion centrally in the growth plate and perpendicular diffusion in the hypertrophic zone, as verified by histology and micro-CT. Conclusion: MRI-DTI may be useful for evaluating the growth plates.
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- author
- Kvist, Ola ; Damberg, Peter ; Dou, Zelong ; Berglund, Johan Sanmartin ; Flodmark, Carl Erik LU ; Nilsson, Ola and Diaz, Sandra LU
- organization
- publishing date
- 2023-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cartilage, diffusion-tensor imaging (DTI), growth plate, magnetic resonance imaging (MRI), skeletal maturation
- in
- Magnetic Resonance in Medicine
- volume
- 89
- issue
- 1
- pages
- 12 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:36110062
- scopus:85138045112
- ISSN
- 0740-3194
- DOI
- 10.1002/mrm.29432
- language
- English
- LU publication?
- yes
- id
- dc33b909-d2f2-4487-a025-154cf73cf5c3
- date added to LUP
- 2022-12-05 09:19:44
- date last changed
- 2024-05-02 18:45:10
@article{dc33b909-d2f2-4487-a025-154cf73cf5c3,
abstract = {{<p>Purpose: To assess the ability of MRI-DTI to evaluate growth plate morphology and activity compared with that of histomorphometry and micro-CT in rabbits. Methods: The hind limbs of female rabbits aged 16, 20, and 24 wk (n = 4 per age group) were studied using a 9.4T MRI scanner with a multi-gradient echo 3D sequence and DTI in 14 directions (b-value = 984 s/mm<sup>2</sup>). After MRI, the right and left hind limb were processed for histological analysis and micro-CT, respectively. The Wilcoxon signed-rank test was used to evaluate the height and volume of the growth plate. Intraclass correlation and Pearson correlation coefficient were used to evaluate the association between DTI metrics and age. Results: The growth plate height and volume were similar for all modalities at each time point and age. Age was correlated with all tractography and DTI metrics in both the femur and tibia. A correlation was also observed between all the metrics at both sites. Tract number and volume declined with age; however, tract length did not show any changes. The fractional anisotropy color map showed lateral diffusion centrally in the growth plate and perpendicular diffusion in the hypertrophic zone, as verified by histology and micro-CT. Conclusion: MRI-DTI may be useful for evaluating the growth plates.</p>}},
author = {{Kvist, Ola and Damberg, Peter and Dou, Zelong and Berglund, Johan Sanmartin and Flodmark, Carl Erik and Nilsson, Ola and Diaz, Sandra}},
issn = {{0740-3194}},
keywords = {{cartilage; diffusion-tensor imaging (DTI); growth plate; magnetic resonance imaging (MRI); skeletal maturation}},
language = {{eng}},
number = {{1}},
pages = {{331--342}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Magnetic Resonance in Medicine}},
title = {{Magnetic resonance and diffusion tensor imaging of the adolescent rabbit growth plate of the knee}},
url = {{http://dx.doi.org/10.1002/mrm.29432}},
doi = {{10.1002/mrm.29432}},
volume = {{89}},
year = {{2023}},
}