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Structural and quantitative comparison of cerebrospinal fluid glycoproteins in Alzheimer's disease patients and healthy individuals

Sihlbom, Carina; Davidsson, Pia; Sjögren, Magnus; Wahlund, Lars -Olof and Nilsson, Carol L LU (2008) In Neurochemical Research 33(7). p.1332-1340
Abstract

Glycoproteins in cerebrospinal fluid (CSF) are altered in Alzheimer's Disease (AD) patients compared to control individuals. We have utilized albumin depletion prior to 2D gel electrophoresis to enhance glycoprotein concentration for image analysis as well as structural glycoprotein determination without glycan release using mass spectrometry (MS). The benefits of a direct glycoprotein analysis approach include minimal sample manipulation and retention of structural details. A quantitative comparison of gel-separated glycoprotein isoforms from twelve AD patients and twelve control subjects was performed with glycoprotein-specific and total protein stains. We have also compared glycoforms in pooled CSF obtained from AD patients and... (More)

Glycoproteins in cerebrospinal fluid (CSF) are altered in Alzheimer's Disease (AD) patients compared to control individuals. We have utilized albumin depletion prior to 2D gel electrophoresis to enhance glycoprotein concentration for image analysis as well as structural glycoprotein determination without glycan release using mass spectrometry (MS). The benefits of a direct glycoprotein analysis approach include minimal sample manipulation and retention of structural details. A quantitative comparison of gel-separated glycoprotein isoforms from twelve AD patients and twelve control subjects was performed with glycoprotein-specific and total protein stains. We have also compared glycoforms in pooled CSF obtained from AD patients and control subjects with mass spectrometry. One isoform of alpha1-antitrypsin showed decreased glycosylation in AD patients while another glycosylated isoform of an unassigned protein was up-regulated. Protein expression levels of alpha1-antitrypsin were decreased, while the protein levels of apolipoprotein E and clusterin were increased in AD. No specific glycoform could be specifically assigned to AD.

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keywords
Adult, Aged, Aged, 80 and over, Albumins, Alzheimer Disease, Chromatography, Affinity, Electrophoresis, Gel, Two-Dimensional, Female, Glycoproteins, Humans, Hydrogen-Ion Concentration, Image Processing, Computer-Assisted, Male, Mass Spectrometry, Middle Aged, Spectroscopy, Fourier Transform Infrared, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Neurochemical Research
volume
33
issue
7
pages
9 pages
publisher
Springer
external identifiers
  • scopus:44549087658
ISSN
0364-3190
DOI
10.1007/s11064-008-9588-x
language
English
LU publication?
no
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dcce551d-cea0-4648-afac-42ce33049d95
date added to LUP
2017-05-16 10:31:33
date last changed
2017-11-14 09:51:44
@article{dcce551d-cea0-4648-afac-42ce33049d95,
  abstract     = {<p>Glycoproteins in cerebrospinal fluid (CSF) are altered in Alzheimer's Disease (AD) patients compared to control individuals. We have utilized albumin depletion prior to 2D gel electrophoresis to enhance glycoprotein concentration for image analysis as well as structural glycoprotein determination without glycan release using mass spectrometry (MS). The benefits of a direct glycoprotein analysis approach include minimal sample manipulation and retention of structural details. A quantitative comparison of gel-separated glycoprotein isoforms from twelve AD patients and twelve control subjects was performed with glycoprotein-specific and total protein stains. We have also compared glycoforms in pooled CSF obtained from AD patients and control subjects with mass spectrometry. One isoform of alpha1-antitrypsin showed decreased glycosylation in AD patients while another glycosylated isoform of an unassigned protein was up-regulated. Protein expression levels of alpha1-antitrypsin were decreased, while the protein levels of apolipoprotein E and clusterin were increased in AD. No specific glycoform could be specifically assigned to AD.</p>},
  author       = {Sihlbom, Carina and Davidsson, Pia and Sjögren, Magnus and Wahlund, Lars -Olof and Nilsson, Carol L},
  issn         = {0364-3190},
  keyword      = {Adult,Aged,Aged, 80 and over,Albumins,Alzheimer Disease,Chromatography, Affinity,Electrophoresis, Gel, Two-Dimensional,Female,Glycoproteins,Humans,Hydrogen-Ion Concentration,Image Processing, Computer-Assisted,Male,Mass Spectrometry,Middle Aged,Spectroscopy, Fourier Transform Infrared,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {7},
  pages        = {1332--1340},
  publisher    = {Springer},
  series       = {Neurochemical Research},
  title        = {Structural and quantitative comparison of cerebrospinal fluid glycoproteins in Alzheimer's disease patients and healthy individuals},
  url          = {http://dx.doi.org/10.1007/s11064-008-9588-x},
  volume       = {33},
  year         = {2008},
}