Advanced

A genome-wide set of 106 microsatellite markers for the blue tit (Cyanistes caeruleus)

Olano-Marin, Juanita; Dawson, Deborah A.; Girg, Alexander; Hansson, Bengt LU ; Ljungqvist, Marcus LU ; Kempenaers, Bart and Mueller, Jakob C. (2010) In Molecular Ecology Resources 10(3). p.516-532
Abstract
We have characterized a set of 106 microsatellite markers in 26-127 individual blue tits (Cyanistes caeruleus), and assigned their location on the zebra finch (Taeniopygia guttata) and on the chicken (Gallus gallus) genome on the basis of sequence homology. Thirty-one markers are newly designed from zebra finch EST (expressed sequence tags) sequences, 22 markers were developed by others from EST sequences using different methods and the remaining 53 loci were previously designed or modified passerine markers. The 106 microsatellite markers are distributed over 26 and 24 chromosomes in the zebra finch and in the chicken genome respectively and the number of alleles varies between 2 and 49. Eight loci deviate significantly from... (More)
We have characterized a set of 106 microsatellite markers in 26-127 individual blue tits (Cyanistes caeruleus), and assigned their location on the zebra finch (Taeniopygia guttata) and on the chicken (Gallus gallus) genome on the basis of sequence homology. Thirty-one markers are newly designed from zebra finch EST (expressed sequence tags) sequences, 22 markers were developed by others from EST sequences using different methods and the remaining 53 loci were previously designed or modified passerine markers. The 106 microsatellite markers are distributed over 26 and 24 chromosomes in the zebra finch and in the chicken genome respectively and the number of alleles varies between 2 and 49. Eight loci deviate significantly from Hardy-Weinberg equilibrium and show a high frequency of null alleles, and three pairs of markers located in the same chromosome appear to be in linkage disequilibrium. With the exception of these few loci, the polymorphic microsatellite markers presented here provide a useful genome-wide resource for population and evolutionary genetic studies of the blue tit, in addition to their potential utility in other passerine birds. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aves, genome map, passerine, microsatellite, blue tit, polymorphism
in
Molecular Ecology Resources
volume
10
issue
3
pages
516 - 532
publisher
Wiley-Blackwell
external identifiers
  • wos:000276407300012
  • scopus:77953080877
ISSN
1755-098X
DOI
10.1111/j.1755-0998.2009.02777.x
language
English
LU publication?
yes
id
dd85fd30-c9f2-49df-898a-85bbfae04eb6 (old id 1603707)
date added to LUP
2010-05-17 14:17:18
date last changed
2018-12-09 03:05:40
@article{dd85fd30-c9f2-49df-898a-85bbfae04eb6,
  abstract     = {We have characterized a set of 106 microsatellite markers in 26-127 individual blue tits (Cyanistes caeruleus), and assigned their location on the zebra finch (Taeniopygia guttata) and on the chicken (Gallus gallus) genome on the basis of sequence homology. Thirty-one markers are newly designed from zebra finch EST (expressed sequence tags) sequences, 22 markers were developed by others from EST sequences using different methods and the remaining 53 loci were previously designed or modified passerine markers. The 106 microsatellite markers are distributed over 26 and 24 chromosomes in the zebra finch and in the chicken genome respectively and the number of alleles varies between 2 and 49. Eight loci deviate significantly from Hardy-Weinberg equilibrium and show a high frequency of null alleles, and three pairs of markers located in the same chromosome appear to be in linkage disequilibrium. With the exception of these few loci, the polymorphic microsatellite markers presented here provide a useful genome-wide resource for population and evolutionary genetic studies of the blue tit, in addition to their potential utility in other passerine birds.},
  author       = {Olano-Marin, Juanita and Dawson, Deborah A. and Girg, Alexander and Hansson, Bengt and Ljungqvist, Marcus and Kempenaers, Bart and Mueller, Jakob C.},
  issn         = {1755-098X},
  keyword      = {Aves,genome map,passerine,microsatellite,blue tit,polymorphism},
  language     = {eng},
  number       = {3},
  pages        = {516--532},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology Resources},
  title        = {A genome-wide set of 106 microsatellite markers for the blue tit (Cyanistes caeruleus)},
  url          = {http://dx.doi.org/10.1111/j.1755-0998.2009.02777.x},
  volume       = {10},
  year         = {2010},
}