Quantitation of protein A in human plasma is possible after heat inactivation of the samples
(1990) In Journal of Immunological Methods 135(1-2). p.77-80- Abstract
- Quantitative determination of staphylococcal protein A in plasma is often hampered by the interaction between protein A and immunoglobulins. In human plasma, these interactions may not only involve the non-immune binding to the Fc or Fab regions of Ig but also antigen/antibody interaction by specific antibodies directed against protein A. In this paper we describe a method which can be used to quantitate nanogram amounts of protein A in the presence of human plasma. The ELISA used for the quantification of protein A is based on a double antibody solid-phase assay utilizing chicken anti-protein A as both capture and detector antibody. Protein A may be measured down to 5 ng/ml in plasma and 0.5 ng/ml in buffer. The plasma samples were... (More)
- Quantitative determination of staphylococcal protein A in plasma is often hampered by the interaction between protein A and immunoglobulins. In human plasma, these interactions may not only involve the non-immune binding to the Fc or Fab regions of Ig but also antigen/antibody interaction by specific antibodies directed against protein A. In this paper we describe a method which can be used to quantitate nanogram amounts of protein A in the presence of human plasma. The ELISA used for the quantification of protein A is based on a double antibody solid-phase assay utilizing chicken anti-protein A as both capture and detector antibody. Protein A may be measured down to 5 ng/ml in plasma and 0.5 ng/ml in buffer. The plasma samples were heat-inactivated before analysis and this eliminated interference in the assay caused by interaction of protein A with an excess of immunoglobulins. This assay provides a reliable and convenient method for the detection and quantitation of soluble protein A in human plasma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105128
- author
- Nilsson, Rune LU and Davidsson, Bertil
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Protein A, ELISA, Plasma, human, Anti-protein A, Heat denaturation
- in
- Journal of Immunological Methods
- volume
- 135
- issue
- 1-2
- pages
- 77 - 80
- publisher
- Elsevier
- external identifiers
-
- pmid:2273267
- scopus:0025633213
- ISSN
- 1872-7905
- DOI
- 10.1016/0022-1759(90)90258-W
- language
- English
- LU publication?
- no
- id
- dd942e36-2f73-4cfd-b35d-22b374210092 (old id 1105128)
- date added to LUP
- 2016-04-01 16:58:12
- date last changed
- 2021-01-03 07:03:34
@article{dd942e36-2f73-4cfd-b35d-22b374210092,
abstract = {{Quantitative determination of staphylococcal protein A in plasma is often hampered by the interaction between protein A and immunoglobulins. In human plasma, these interactions may not only involve the non-immune binding to the Fc or Fab regions of Ig but also antigen/antibody interaction by specific antibodies directed against protein A. In this paper we describe a method which can be used to quantitate nanogram amounts of protein A in the presence of human plasma. The ELISA used for the quantification of protein A is based on a double antibody solid-phase assay utilizing chicken anti-protein A as both capture and detector antibody. Protein A may be measured down to 5 ng/ml in plasma and 0.5 ng/ml in buffer. The plasma samples were heat-inactivated before analysis and this eliminated interference in the assay caused by interaction of protein A with an excess of immunoglobulins. This assay provides a reliable and convenient method for the detection and quantitation of soluble protein A in human plasma.}},
author = {{Nilsson, Rune and Davidsson, Bertil}},
issn = {{1872-7905}},
keywords = {{Protein A; ELISA; Plasma; human; Anti-protein A; Heat denaturation}},
language = {{eng}},
number = {{1-2}},
pages = {{77--80}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{Quantitation of protein A in human plasma is possible after heat inactivation of the samples}},
url = {{http://dx.doi.org/10.1016/0022-1759(90)90258-W}},
doi = {{10.1016/0022-1759(90)90258-W}},
volume = {{135}},
year = {{1990}},
}