The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology
(2009) In Journal of Applied Biomaterials and Biomechanics 7(1). p.13-22- Abstract
- Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot... (More)
- Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22) (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1477033
- author
- Sandin, Karin ; Kloo, Lars ; Odselius, Rolf LU and Olsson, Lars-Fride
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nano-crystalline, Mineralization, Apatite, Carbonated apatite
- in
- Journal of Applied Biomaterials and Biomechanics
- volume
- 7
- issue
- 1
- pages
- 13 - 22
- publisher
- Wichtig Editore
- external identifiers
-
- wos:000269103400002
- scopus:68149130919
- ISSN
- 1724-6024
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Electron Microscopy Unit (013100002)
- id
- ddae3f90-788f-40a6-87a0-869504588b2c (old id 1477033)
- date added to LUP
- 2016-04-01 11:59:41
- date last changed
- 2022-04-21 00:52:41
@article{ddae3f90-788f-40a6-87a0-869504588b2c,
abstract = {{Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22)}},
author = {{Sandin, Karin and Kloo, Lars and Odselius, Rolf and Olsson, Lars-Fride}},
issn = {{1724-6024}},
keywords = {{Nano-crystalline; Mineralization; Apatite; Carbonated apatite}},
language = {{eng}},
number = {{1}},
pages = {{13--22}},
publisher = {{Wichtig Editore}},
series = {{Journal of Applied Biomaterials and Biomechanics}},
title = {{The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology}},
volume = {{7}},
year = {{2009}},
}