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Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease

Chumarina, Margarita LU ; Azevedo, Carla LU ; Bigarreau, Julie; Vignal, Clémentine; Kim, Kwang Soo; Li, Jia-Yi LU and Roybon, Laurent LU (2017) In Stem Cell Research 19. p.17-20
Abstract

Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.

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author
organization
publishing date
type
Contribution to journal
publication status
published
in
Stem Cell Research
volume
19
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85020318540
  • wos:000397450700004
ISSN
1876-7753
DOI
10.1016/j.scr.2016.12.026
language
English
LU publication?
yes
id
de3f6c47-6130-4233-a0da-2b7f871f8a59
date added to LUP
2017-08-21 11:56:03
date last changed
2017-09-18 11:42:21
@article{de3f6c47-6130-4233-a0da-2b7f871f8a59,
  abstract     = {<p>Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.</p>},
  author       = {Chumarina, Margarita and Azevedo, Carla and Bigarreau, Julie and Vignal, Clémentine and Kim, Kwang Soo and Li, Jia-Yi and Roybon, Laurent},
  issn         = {1876-7753},
  language     = {eng},
  month        = {03},
  pages        = {17--20},
  publisher    = {Elsevier},
  series       = {Stem Cell Research},
  title        = {Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease},
  url          = {http://dx.doi.org/10.1016/j.scr.2016.12.026},
  volume       = {19},
  year         = {2017},
}