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CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS

Goyal, Gaurav ; Ekedahl, Elina ; Nyblom, My ; Krog, Jens LU ; Torstensson, Erik ; Sewunet, Tsegaye ; Giske, Christian LU ; Thamlikitkul, Visanu ; Ambjörnsson, Tobias LU and Westerlund, Fredrik (2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.801-802
Abstract

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Anti-microbial resistance, CRISPR/Cas9, DNA, DNA combing, Plasmid, Single-molecule
host publication
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
conference location
Palm Springs, Virtual, United States
conference dates
2021-10-10 - 2021-10-14
external identifiers
  • scopus:85136934697
ISBN
9781733419031
language
English
LU publication?
yes
id
de441d21-8de0-4cd5-b9bf-d55597eed89a
date added to LUP
2022-11-08 10:53:38
date last changed
2024-03-21 06:35:14
@inproceedings{de441d21-8de0-4cd5-b9bf-d55597eed89a,
  abstract     = {{<p>We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.</p>}},
  author       = {{Goyal, Gaurav and Ekedahl, Elina and Nyblom, My and Krog, Jens and Torstensson, Erik and Sewunet, Tsegaye and Giske, Christian and Thamlikitkul, Visanu and Ambjörnsson, Tobias and Westerlund, Fredrik}},
  booktitle    = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419031}},
  keywords     = {{Anti-microbial resistance; CRISPR/Cas9; DNA; DNA combing; Plasmid; Single-molecule}},
  language     = {{eng}},
  pages        = {{801--802}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS}},
  year         = {{2021}},
}