CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS
(2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.801-802- Abstract
We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/de441d21-8de0-4cd5-b9bf-d55597eed89a
- author
- Goyal, Gaurav ; Ekedahl, Elina ; Nyblom, My ; Krog, Jens LU ; Torstensson, Erik ; Sewunet, Tsegaye ; Giske, Christian LU ; Thamlikitkul, Visanu ; Ambjörnsson, Tobias LU and Westerlund, Fredrik
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Anti-microbial resistance, CRISPR/Cas9, DNA, DNA combing, Plasmid, Single-molecule
- host publication
- MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
- conference location
- Palm Springs, Virtual, United States
- conference dates
- 2021-10-10 - 2021-10-14
- external identifiers
-
- scopus:85136934697
- ISBN
- 9781733419031
- language
- English
- LU publication?
- yes
- id
- de441d21-8de0-4cd5-b9bf-d55597eed89a
- date added to LUP
- 2022-11-08 10:53:38
- date last changed
- 2024-03-21 06:35:14
@inproceedings{de441d21-8de0-4cd5-b9bf-d55597eed89a, abstract = {{<p>We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.</p>}}, author = {{Goyal, Gaurav and Ekedahl, Elina and Nyblom, My and Krog, Jens and Torstensson, Erik and Sewunet, Tsegaye and Giske, Christian and Thamlikitkul, Visanu and Ambjörnsson, Tobias and Westerlund, Fredrik}}, booktitle = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419031}}, keywords = {{Anti-microbial resistance; CRISPR/Cas9; DNA; DNA combing; Plasmid; Single-molecule}}, language = {{eng}}, pages = {{801--802}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS}}, year = {{2021}}, }