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Exploration of physical and chemical cues on retinal cell fate

Zalis, Marina Castro LU ; Johansson, Sebastian; Johansson, Fredrik LU and Johansson, Ulrica Englund LU (2016) In Molecular and Cellular Neuroscience 75. p.122-132
Abstract

Identification of the key components in the physical and chemical milieu directing donor cells into a desired phenotype is a requirement in the investigation of bioscaffolds for the advancement of cell-based therapies for retinal neurodegeneration. We explore the effect of electrospun poly-ε-caprolactone (PCL) fiber scaffold topography and functionalization and culture medium, on the behavior of mouse retinal cells. Dissociated mouse retinal post-natal cells were seeded on random or aligned oriented fibers, with or without laminin coating and cultured with either basic or neurotrophins enriched medium for 7 days. Addition of laminin in combination with neurotrophins clearly promoted cell– morphology, fate, and neurite extension.... (More)

Identification of the key components in the physical and chemical milieu directing donor cells into a desired phenotype is a requirement in the investigation of bioscaffolds for the advancement of cell-based therapies for retinal neurodegeneration. We explore the effect of electrospun poly-ε-caprolactone (PCL) fiber scaffold topography and functionalization and culture medium, on the behavior of mouse retinal cells. Dissociated mouse retinal post-natal cells were seeded on random or aligned oriented fibers, with or without laminin coating and cultured with either basic or neurotrophins enriched medium for 7 days. Addition of laminin in combination with neurotrophins clearly promoted cell– morphology, fate, and neurite extension. Nanotopography per se significantly affected cell morphology, with mainly bipolar profiles on aligned fibers and more multipolar profiles on random fibers. Laminin induced a remarkable 90° switch of neurite orientation. Herewith, we demonstrate that the chemical cue is stronger than the physical cue for the orientation of retinal neurites and describe the requirement of both neurotrophins and extracellular matrix proteins for extended neurite outgrowth and formation of complex retinal neuronal networks. Therefore, tailor-made PCL fiber mats, which can be physically and chemically modified, indeed influence cell behavior and hence motivate further retinal restorative studies using this system.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Aligned and random nanofibrous scaffolds, Laminin, Neurotrophins, Poly-ε-caprolactone, Post-natal retinal cells, Retinal tissue engineering
in
Molecular and Cellular Neuroscience
volume
75
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:84981320361
  • wos:000382422600013
ISSN
1044-7431
DOI
10.1016/j.mcn.2016.07.006
language
English
LU publication?
yes
id
de4dfcb8-8cff-479e-915e-bc8a8dc620bb
date added to LUP
2016-11-11 15:19:40
date last changed
2017-05-21 04:55:28
@article{de4dfcb8-8cff-479e-915e-bc8a8dc620bb,
  abstract     = {<p>Identification of the key components in the physical and chemical milieu directing donor cells into a desired phenotype is a requirement in the investigation of bioscaffolds for the advancement of cell-based therapies for retinal neurodegeneration. We explore the effect of electrospun poly-ε-caprolactone (PCL) fiber scaffold topography and functionalization and culture medium, on the behavior of mouse retinal cells. Dissociated mouse retinal post-natal cells were seeded on random or aligned oriented fibers, with or without laminin coating and cultured with either basic or neurotrophins enriched medium for 7 days. Addition of laminin in combination with neurotrophins clearly promoted cell– morphology, fate, and neurite extension. Nanotopography per se significantly affected cell morphology, with mainly bipolar profiles on aligned fibers and more multipolar profiles on random fibers. Laminin induced a remarkable 90° switch of neurite orientation. Herewith, we demonstrate that the chemical cue is stronger than the physical cue for the orientation of retinal neurites and describe the requirement of both neurotrophins and extracellular matrix proteins for extended neurite outgrowth and formation of complex retinal neuronal networks. Therefore, tailor-made PCL fiber mats, which can be physically and chemically modified, indeed influence cell behavior and hence motivate further retinal restorative studies using this system.</p>},
  author       = {Zalis, Marina Castro and Johansson, Sebastian and Johansson, Fredrik and Johansson, Ulrica Englund},
  issn         = {1044-7431},
  keyword      = {Aligned and random nanofibrous scaffolds,Laminin,Neurotrophins,Poly-ε-caprolactone,Post-natal retinal cells,Retinal tissue engineering},
  language     = {eng},
  month        = {09},
  pages        = {122--132},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Exploration of physical and chemical cues on retinal cell fate},
  url          = {http://dx.doi.org/10.1016/j.mcn.2016.07.006},
  volume       = {75},
  year         = {2016},
}