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A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli

Gonzalez de Valdivia, Ernesto I LU and Isaksson, Leif A (2004) In Nucleic Acids Research 32(17). p.205-5198
Abstract

The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA... (More)

The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Codon, Codon, Initiator, Escherichia coli, Gene Expression Regulation, Bacterial, Protein Biosynthesis, RNA, Messenger, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
in
Nucleic Acids Research
volume
32
issue
17
pages
8 pages
publisher
Oxford University Press
external identifiers
  • scopus:4944224262
ISSN
1362-4962
DOI
10.1093/nar/gkh857
language
English
LU publication?
no
id
de6ca128-34e6-49fc-974a-9b5661cd07aa
date added to LUP
2018-01-13 11:56:15
date last changed
2018-11-21 21:37:18
@article{de6ca128-34e6-49fc-974a-9b5661cd07aa,
  abstract     = {<p>The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.</p>},
  author       = {Gonzalez de Valdivia, Ernesto I and Isaksson, Leif A},
  issn         = {1362-4962},
  keyword      = {Codon,Codon, Initiator,Escherichia coli,Gene Expression Regulation, Bacterial,Protein Biosynthesis,RNA, Messenger,Transcription, Genetic,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {17},
  pages        = {205--5198},
  publisher    = {Oxford University Press},
  series       = {Nucleic Acids Research},
  title        = {A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli},
  url          = {http://dx.doi.org/10.1093/nar/gkh857},
  volume       = {32},
  year         = {2004},
}