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Dynamics and MicroRNA144/451-mediated mechanisms of regulatory B Cells during Schistosoma japonicum infection in Mice

Tian, Fang ; Jiao, Yumeng ; Yang, Bin LU ; Xian, Kangwen ; Xu, Mengqi ; Xu, Jiahui ; Song, Lijun ; Qian, Li ; Han, Jin Hee and Han, Eun Taek , et al. (2025) In Acta Tropica 272.
Abstract

Schistosoma japonicum (S. japonicum) infection and soluble egg antigens (SEA) can induce various immune responses, including the generation of IL-10-producing regulatory B cells (Bregs). This study investigated the dynamic changes and induction mechanisms of Bregs during S. japonicum infection. We demonstrated that CD19+IL-10+Bregs and CD19+Tim-1+ Bregs secreting IL-10 gradually increased from 6 to 13 weeks after infection. The CD19+Tim-1+ Bregs exhibited immunosuppressive functions by promoting IL-4 secretion, inhibiting IL-17 production in CD4+ T cells, and increasing the percentages of CD4+CD25+Foxp3+ T cells. MicroRNAs play... (More)

Schistosoma japonicum (S. japonicum) infection and soluble egg antigens (SEA) can induce various immune responses, including the generation of IL-10-producing regulatory B cells (Bregs). This study investigated the dynamic changes and induction mechanisms of Bregs during S. japonicum infection. We demonstrated that CD19+IL-10+Bregs and CD19+Tim-1+ Bregs secreting IL-10 gradually increased from 6 to 13 weeks after infection. The CD19+Tim-1+ Bregs exhibited immunosuppressive functions by promoting IL-4 secretion, inhibiting IL-17 production in CD4+ T cells, and increasing the percentages of CD4+CD25+Foxp3+ T cells. MicroRNAs play a key role in both the early differentiation and effector differentiation of B cells in the immune system. We analyzed the expression of miRNAs in the splenic B cells of mice infected with S. japonicum for 13 weeks and predicted their target genes. There were 33 up-regulated and 10 down-regulated miRNAs. Furthermore, we found that Bregs could not be induced in miR-144/451-/- mice after S. japonicum infection and SEA stimulation. The effect of miR-144/451 on Bregs may be mediated through the mTOR signaling pathway. These findings provide new insights into the mechanisms underlying Bregs activation and their role in modulating immune responses during schistosomiasis.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
miR-144/451, Regulatory B cells, Schistosoma japonicum
in
Acta Tropica
volume
272
article number
107912
publisher
Elsevier
external identifiers
  • pmid:41242644
  • scopus:105022160459
ISSN
0001-706X
DOI
10.1016/j.actatropica.2025.107912
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2025 Elsevier B.V.
id
def00982-4f29-46ac-a06a-9ee4da7536ee
date added to LUP
2026-01-16 15:50:52
date last changed
2026-01-19 14:22:28
@article{def00982-4f29-46ac-a06a-9ee4da7536ee,
  abstract     = {{<p>Schistosoma japonicum (S. japonicum) infection and soluble egg antigens (SEA) can induce various immune responses, including the generation of IL-10-producing regulatory B cells (Bregs). This study investigated the dynamic changes and induction mechanisms of Bregs during S. japonicum infection. We demonstrated that CD19<sup>+</sup>IL-10<sup>+</sup>Bregs and CD19<sup>+</sup>Tim-1<sup>+</sup> Bregs secreting IL-10 gradually increased from 6 to 13 weeks after infection. The CD19<sup>+</sup>Tim-1<sup>+</sup> Bregs exhibited immunosuppressive functions by promoting IL-4 secretion, inhibiting IL-17 production in CD4<sup>+</sup> T cells, and increasing the percentages of CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> T cells. MicroRNAs play a key role in both the early differentiation and effector differentiation of B cells in the immune system. We analyzed the expression of miRNAs in the splenic B cells of mice infected with S. japonicum for 13 weeks and predicted their target genes. There were 33 up-regulated and 10 down-regulated miRNAs. Furthermore, we found that Bregs could not be induced in miR-144/451<sup>-/-</sup> mice after S. japonicum infection and SEA stimulation. The effect of miR-144/451 on Bregs may be mediated through the mTOR signaling pathway. These findings provide new insights into the mechanisms underlying Bregs activation and their role in modulating immune responses during schistosomiasis.</p>}},
  author       = {{Tian, Fang and Jiao, Yumeng and Yang, Bin and Xian, Kangwen and Xu, Mengqi and Xu, Jiahui and Song, Lijun and Qian, Li and Han, Jin Hee and Han, Eun Taek and Lu, Feng}},
  issn         = {{0001-706X}},
  keywords     = {{miR-144/451; Regulatory B cells; Schistosoma japonicum}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Acta Tropica}},
  title        = {{Dynamics and MicroRNA144/451-mediated mechanisms of regulatory B Cells during Schistosoma japonicum infection in Mice}},
  url          = {{http://dx.doi.org/10.1016/j.actatropica.2025.107912}},
  doi          = {{10.1016/j.actatropica.2025.107912}},
  volume       = {{272}},
  year         = {{2025}},
}