Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter
Bækkeskov, Steinunn and Lernmark, Åke LU
(1983)
In FEBS Letters
157(2).
p.331-335
- Abstract
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36 000 and a minor protein of Mr 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding... (More)
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36 000 and a minor protein of Mr 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.
(Less)
- author
- Bækkeskov, Steinunn
and Lernmark, Åke
LU
- publishing date
- 1983-07-04
- type
- Contribution to journal
- publication status
- published
- keywords
- 3-Isobutyl-1-methylxanthine, Glucose regulation, Glucose transport, Human erythrocyte glucose transporter, Human pancreatic islet, β-Cell protein
- in
- FEBS Letters
- volume
- 157
- issue
- 2
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:6190679
- scopus:0020597231
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(83)80570-5
- language
- English
- LU publication?
- no
- id
- df0589fd-8acc-4e58-b2fe-b91a63fe6bef
- date added to LUP
- 2019-09-16 12:49:12
- date last changed
- 2024-03-13 08:07:26
@article{df0589fd-8acc-4e58-b2fe-b91a63fe6bef,
abstract = {{<p>An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of M<sub>r</sub>36 000 and a minor protein of M<sub>r</sub> 58 000 from human pancreatic islets. The biosynthetic incorporation of [<sup>35</sup>S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.</p>}},
author = {{Bækkeskov, Steinunn and Lernmark, Åke}},
issn = {{0014-5793}},
keywords = {{3-Isobutyl-1-methylxanthine; Glucose regulation; Glucose transport; Human erythrocyte glucose transporter; Human pancreatic islet; β-Cell protein}},
language = {{eng}},
month = {{07}},
number = {{2}},
pages = {{331--335}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter}},
url = {{http://dx.doi.org/10.1016/0014-5793(83)80570-5}},
doi = {{10.1016/0014-5793(83)80570-5}},
volume = {{157}},
year = {{1983}},
}