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Two types of muscarinic acetylcholine receptors in Drosophila and other arthropods

Collin, Caitlin; Hauser, Frank; de Valdivia, Ernesto Gonzalez LU ; Li, Shizhong; Reisenberger, Julia; Carlsen, Eva M M; Khan, Zaid; Hansen, Niels O; Puhm, Florian and Søndergaard, Leif, et al. (2013) In Cellular and Molecular Life Sciences1997-01-01+01:00 70(17). p.42-3231
Abstract

Muscarinic acetylcholine receptors (mAChRs) play a central role in the mammalian nervous system. These receptors are G protein-coupled receptors (GPCRs), which are activated by the agonists acetylcholine and muscarine, and blocked by a variety of antagonists. Mammals have five mAChRs (m1-m5). In this study, we cloned two structurally related GPCRs from the fruit fly Drosophila melanogaster, which, after expression in Chinese hamster ovary cells, proved to be muscarinic acetylcholine receptors. One mAChR (the A-type; encoded by gene CG4356) is activated by acetylcholine (EC50, 5 × 10(-8) M) and muscarine (EC50, 6 × 10(-8) M) and blocked by the classical mAChR antagonists atropine, scopolamine, and 3-quinuclidinyl-benzilate (QNB), while... (More)

Muscarinic acetylcholine receptors (mAChRs) play a central role in the mammalian nervous system. These receptors are G protein-coupled receptors (GPCRs), which are activated by the agonists acetylcholine and muscarine, and blocked by a variety of antagonists. Mammals have five mAChRs (m1-m5). In this study, we cloned two structurally related GPCRs from the fruit fly Drosophila melanogaster, which, after expression in Chinese hamster ovary cells, proved to be muscarinic acetylcholine receptors. One mAChR (the A-type; encoded by gene CG4356) is activated by acetylcholine (EC50, 5 × 10(-8) M) and muscarine (EC50, 6 × 10(-8) M) and blocked by the classical mAChR antagonists atropine, scopolamine, and 3-quinuclidinyl-benzilate (QNB), while the other (the B-type; encoded by gene CG7918) is also activated by acetylcholine, but has a 1,000-fold lower sensitivity to muscarine, and is not blocked by the antagonists. A- and B-type mAChRs were also cloned and functionally characterized from the red flour beetle Tribolium castaneum. Recently, Haga et al. (Nature 2012, 482: 547-551) published the crystal structure of the human m2 mAChR, revealing 14 amino acid residues forming the binding pocket for QNB. These residues are identical between the human m2 and the D. melanogaster and T. castaneum A-type mAChRs, while many of them are different between the human m2 and the B-type receptors. Using bioinformatics, one orthologue of the A-type and one of the B-type mAChRs could also be found in all other arthropods with a sequenced genome. Protostomes, such as arthropods, and deuterostomes, such as mammals and other vertebrates, belong to two evolutionarily distinct lineages of animal evolution that split about 700 million years ago. We found that animals that originated before this split, such as cnidarians (Hydra), had two A-type mAChRs. From these data we propose a model for the evolution of mAChRs.

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keywords
Animals, Animals, Genetically Modified, Arthropods, Base Sequence, CHO Cells, Cricetinae, Cricetulus, Down-Regulation, Drosophila, Humans, Molecular Sequence Data, RNA, Messenger, Receptors, Muscarinic, Journal Article, Research Support, Non-U.S. Gov't
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Cellular and Molecular Life Sciences1997-01-01+01:00
volume
70
issue
17
pages
12 pages
publisher
Birkhaüser
external identifiers
  • scopus:84882456615
ISSN
1420-9071
DOI
10.1007/s00018-013-1334-0
language
English
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no
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df0b68f2-1dbb-4e7f-9829-40cd702c0047
date added to LUP
2018-01-12 17:01:11
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2018-07-29 04:32:15
@article{df0b68f2-1dbb-4e7f-9829-40cd702c0047,
  abstract     = {<p>Muscarinic acetylcholine receptors (mAChRs) play a central role in the mammalian nervous system. These receptors are G protein-coupled receptors (GPCRs), which are activated by the agonists acetylcholine and muscarine, and blocked by a variety of antagonists. Mammals have five mAChRs (m1-m5). In this study, we cloned two structurally related GPCRs from the fruit fly Drosophila melanogaster, which, after expression in Chinese hamster ovary cells, proved to be muscarinic acetylcholine receptors. One mAChR (the A-type; encoded by gene CG4356) is activated by acetylcholine (EC50, 5 × 10(-8) M) and muscarine (EC50, 6 × 10(-8) M) and blocked by the classical mAChR antagonists atropine, scopolamine, and 3-quinuclidinyl-benzilate (QNB), while the other (the B-type; encoded by gene CG7918) is also activated by acetylcholine, but has a 1,000-fold lower sensitivity to muscarine, and is not blocked by the antagonists. A- and B-type mAChRs were also cloned and functionally characterized from the red flour beetle Tribolium castaneum. Recently, Haga et al. (Nature 2012, 482: 547-551) published the crystal structure of the human m2 mAChR, revealing 14 amino acid residues forming the binding pocket for QNB. These residues are identical between the human m2 and the D. melanogaster and T. castaneum A-type mAChRs, while many of them are different between the human m2 and the B-type receptors. Using bioinformatics, one orthologue of the A-type and one of the B-type mAChRs could also be found in all other arthropods with a sequenced genome. Protostomes, such as arthropods, and deuterostomes, such as mammals and other vertebrates, belong to two evolutionarily distinct lineages of animal evolution that split about 700 million years ago. We found that animals that originated before this split, such as cnidarians (Hydra), had two A-type mAChRs. From these data we propose a model for the evolution of mAChRs.</p>},
  author       = {Collin, Caitlin and Hauser, Frank and de Valdivia, Ernesto Gonzalez and Li, Shizhong and Reisenberger, Julia and Carlsen, Eva M M and Khan, Zaid and Hansen, Niels O and Puhm, Florian and Søndergaard, Leif and Niemiec, Justyna and Heninger, Magdalena and Ren, Guilin R and Grimmelikhuijzen, Cornelis J P},
  issn         = {1420-9071},
  keyword      = {Animals,Animals, Genetically Modified,Arthropods,Base Sequence,CHO Cells,Cricetinae,Cricetulus,Down-Regulation,Drosophila,Humans,Molecular Sequence Data,RNA, Messenger,Receptors, Muscarinic,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {17},
  pages        = {42--3231},
  publisher    = {Birkhaüser},
  series       = {Cellular and Molecular Life Sciences1997-01-01+01:00},
  title        = {Two types of muscarinic acetylcholine receptors in Drosophila and other arthropods},
  url          = {http://dx.doi.org/10.1007/s00018-013-1334-0},
  volume       = {70},
  year         = {2013},
}