Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury
(2012) In International Journal of Impotence Research 24(2). p.84-90- Abstract
- In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery... (More)
- In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011 (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2515729
- author
- Kutlu, O. ; Ross, A. E. ; Schaeffer, E. M. ; Gratzke, C. ; Stief, C. G. ; Strong, T. D. ; Burnett, A. L. ; Hedlund, Petter LU and Bivalacqua, T. J.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- stem cell, radical prostatectomy, prostate cancer, erectile dysfunction
- in
- International Journal of Impotence Research
- volume
- 24
- issue
- 2
- pages
- 84 - 90
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000301714200005
- scopus:84858704689
- pmid:21993267
- ISSN
- 1476-5489
- DOI
- 10.1038/ijir.2011.50
- language
- English
- LU publication?
- yes
- id
- df406c91-1d09-4c00-af4a-1f3402298912 (old id 2515729)
- date added to LUP
- 2016-04-01 10:10:15
- date last changed
- 2022-02-17 07:20:27
@article{df406c91-1d09-4c00-af4a-1f3402298912, abstract = {{In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011}}, author = {{Kutlu, O. and Ross, A. E. and Schaeffer, E. M. and Gratzke, C. and Stief, C. G. and Strong, T. D. and Burnett, A. L. and Hedlund, Petter and Bivalacqua, T. J.}}, issn = {{1476-5489}}, keywords = {{stem cell; radical prostatectomy; prostate cancer; erectile dysfunction}}, language = {{eng}}, number = {{2}}, pages = {{84--90}}, publisher = {{Nature Publishing Group}}, series = {{International Journal of Impotence Research}}, title = {{Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury}}, url = {{http://dx.doi.org/10.1038/ijir.2011.50}}, doi = {{10.1038/ijir.2011.50}}, volume = {{24}}, year = {{2012}}, }