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Evidence for the mechanisms of zinc uptake by rice using isotope fractionation

Arnold, Tim LU orcid ; Kirk, Guy J.D. ; Wissuwa, Matthias ; Frei, Michael ; Zhao, Fang Jie ; Mason, Thomas F.D. and Weiss, Dominik J. (2010) In Plant, Cell and Environment 33(3). p.370-381
Abstract

In an earlier study, we found that rice (Oryza sativa) grown in nutrient solution well-supplied with Zn preferentially took up light 64Zn over 66Zn, probably as a result of kinetic fractionation in membrane transport processes. Here, we measure isotope fractionation by rice in a submerged Zn-deficient soil with and without Zn fertilizer. We grew the same genotype as in the nutrient solution study plus low-Zn tolerant and intolerant lines from a recombinant inbred population. In contrast to the nutrient solution, in soil with Zn fertilizer we found little or heavy isotopic enrichment in the plants relative to plant-available Zn in the soil, and in soil without Zn fertilizer we found consistently heavy enrichment,... (More)

In an earlier study, we found that rice (Oryza sativa) grown in nutrient solution well-supplied with Zn preferentially took up light 64Zn over 66Zn, probably as a result of kinetic fractionation in membrane transport processes. Here, we measure isotope fractionation by rice in a submerged Zn-deficient soil with and without Zn fertilizer. We grew the same genotype as in the nutrient solution study plus low-Zn tolerant and intolerant lines from a recombinant inbred population. In contrast to the nutrient solution, in soil with Zn fertilizer we found little or heavy isotopic enrichment in the plants relative to plant-available Zn in the soil, and in soil without Zn fertilizer we found consistently heavy enrichment, particularly in the low-Zn tolerant line. These observations are only explicable by complexation of Zn by a complexing agent released from the roots and uptake of the complexed Zn by specific root transporters. We show with a mathematical model that, for realistic rates of secretion of the phytosiderophore deoxymugineic acid (DMA) by rice, and realistic parameters for the Zn-solubilizing effect of DMA in soil, solubilization and uptake by this mechanism is necessary and sufficient to account for the measured Zn uptake and the differences between genotypes.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
DMA, Isotope fractionation, Phytosiderophore, Rice, Solubilization, Stable isotopes, Zinc
in
Plant, Cell and Environment
volume
33
issue
3
pages
12 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:19929899
  • scopus:77949374334
ISSN
0140-7791
DOI
10.1111/j.1365-3040.2009.02085.x
language
English
LU publication?
no
id
df4a0815-efd7-49a5-927d-52f2f1d32864
date added to LUP
2024-10-24 18:38:15
date last changed
2025-05-23 13:55:29
@article{df4a0815-efd7-49a5-927d-52f2f1d32864,
  abstract     = {{<p>In an earlier study, we found that rice (Oryza sativa) grown in nutrient solution well-supplied with Zn preferentially took up light <sup>64</sup>Zn over <sup>66</sup>Zn, probably as a result of kinetic fractionation in membrane transport processes. Here, we measure isotope fractionation by rice in a submerged Zn-deficient soil with and without Zn fertilizer. We grew the same genotype as in the nutrient solution study plus low-Zn tolerant and intolerant lines from a recombinant inbred population. In contrast to the nutrient solution, in soil with Zn fertilizer we found little or heavy isotopic enrichment in the plants relative to plant-available Zn in the soil, and in soil without Zn fertilizer we found consistently heavy enrichment, particularly in the low-Zn tolerant line. These observations are only explicable by complexation of Zn by a complexing agent released from the roots and uptake of the complexed Zn by specific root transporters. We show with a mathematical model that, for realistic rates of secretion of the phytosiderophore deoxymugineic acid (DMA) by rice, and realistic parameters for the Zn-solubilizing effect of DMA in soil, solubilization and uptake by this mechanism is necessary and sufficient to account for the measured Zn uptake and the differences between genotypes.</p>}},
  author       = {{Arnold, Tim and Kirk, Guy J.D. and Wissuwa, Matthias and Frei, Michael and Zhao, Fang Jie and Mason, Thomas F.D. and Weiss, Dominik J.}},
  issn         = {{0140-7791}},
  keywords     = {{DMA; Isotope fractionation; Phytosiderophore; Rice; Solubilization; Stable isotopes; Zinc}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{370--381}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Plant, Cell and Environment}},
  title        = {{Evidence for the mechanisms of zinc uptake by rice using isotope fractionation}},
  url          = {{http://dx.doi.org/10.1111/j.1365-3040.2009.02085.x}},
  doi          = {{10.1111/j.1365-3040.2009.02085.x}},
  volume       = {{33}},
  year         = {{2010}},
}