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CDK2 regulates the NRF1/Ehmt1 axis during meiotic prophase I

Palmer, Nathan ; Talib, S Zakiah A ; Ratnacaram, Chandrahas Koumar ; Low, Diana ; Bisteau, Xavier ; Lee, Joanna Hui Si ; Pfeiffenberger, Elisabeth ; Wollmann, Heike ; Tan, Joel Heng Loong and Wee, Sheena , et al. (2019) In Journal of Cell Biology 218(9). p.2896-2918
Abstract

Meiosis generates four genetically distinct haploid gametes over the course of two reductional cell divisions. Meiotic divisions are characterized by the coordinated deposition and removal of various epigenetic marks. Here we propose that nuclear respiratory factor 1 (NRF1) regulates transcription of euchromatic histone methyltransferase 1 (EHMT1) to ensure normal patterns of H3K9 methylation during meiotic prophase I. We demonstrate that cyclin-dependent kinase (CDK2) can bind to the promoters of a number of genes in male germ cells including that of Ehmt1 through interaction with the NRF1 transcription factor. Our data indicate that CDK2-mediated phosphorylation of NRF1 can occur at two distinct serine residues and negatively... (More)

Meiosis generates four genetically distinct haploid gametes over the course of two reductional cell divisions. Meiotic divisions are characterized by the coordinated deposition and removal of various epigenetic marks. Here we propose that nuclear respiratory factor 1 (NRF1) regulates transcription of euchromatic histone methyltransferase 1 (EHMT1) to ensure normal patterns of H3K9 methylation during meiotic prophase I. We demonstrate that cyclin-dependent kinase (CDK2) can bind to the promoters of a number of genes in male germ cells including that of Ehmt1 through interaction with the NRF1 transcription factor. Our data indicate that CDK2-mediated phosphorylation of NRF1 can occur at two distinct serine residues and negatively regulates NRF1 DNA binding activity in vitro. Furthermore, induced deletion of Cdk2 in spermatocytes results in increased expression of many NRF1 target genes including Ehmt1 We hypothesize that the regulation of NRF1 transcriptional activity by CDK2 may allow the modulation of Ehmt1 expression, therefore controlling the dynamic methylation of H3K9 during meiotic prophase.

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publication status
published
in
Journal of Cell Biology
volume
218
issue
9
pages
2896 - 2918
publisher
Rockefeller University Press
external identifiers
  • pmid:31350280
ISSN
0021-9525
DOI
10.1083/jcb.201903125
language
English
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no
id
df4ada2d-b6d0-4701-bcf9-03e59f2c240a
date added to LUP
2019-09-17 14:42:20
date last changed
2019-12-18 04:06:14
@article{df4ada2d-b6d0-4701-bcf9-03e59f2c240a,
  abstract     = {<p>Meiosis generates four genetically distinct haploid gametes over the course of two reductional cell divisions. Meiotic divisions are characterized by the coordinated deposition and removal of various epigenetic marks. Here we propose that nuclear respiratory factor 1 (NRF1) regulates transcription of euchromatic histone methyltransferase 1 (EHMT1) to ensure normal patterns of H3K9 methylation during meiotic prophase I. We demonstrate that cyclin-dependent kinase (CDK2) can bind to the promoters of a number of genes in male germ cells including that of Ehmt1 through interaction with the NRF1 transcription factor. Our data indicate that CDK2-mediated phosphorylation of NRF1 can occur at two distinct serine residues and negatively regulates NRF1 DNA binding activity in vitro. Furthermore, induced deletion of Cdk2 in spermatocytes results in increased expression of many NRF1 target genes including Ehmt1 We hypothesize that the regulation of NRF1 transcriptional activity by CDK2 may allow the modulation of Ehmt1 expression, therefore controlling the dynamic methylation of H3K9 during meiotic prophase.</p>},
  author       = {Palmer, Nathan and Talib, S Zakiah A and Ratnacaram, Chandrahas Koumar and Low, Diana and Bisteau, Xavier and Lee, Joanna Hui Si and Pfeiffenberger, Elisabeth and Wollmann, Heike and Tan, Joel Heng Loong and Wee, Sheena and Sobota, Radoslaw and Gunaratne, Jayantha and Messerschmidt, Daniel M and Guccione, Ernesto and Kaldis, Philipp},
  issn         = {0021-9525},
  language     = {eng},
  month        = {09},
  number       = {9},
  pages        = {2896--2918},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Cell Biology},
  title        = {CDK2 regulates the NRF1/Ehmt1 axis during meiotic prophase I},
  url          = {http://dx.doi.org/10.1083/jcb.201903125},
  doi          = {10.1083/jcb.201903125},
  volume       = {218},
  year         = {2019},
}