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Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP

Van Der Plas, M. J.A. LU ; Baldry, M.; Van Dissel, J. T.; Jukema, G. N. and Nibbering, P. H. (2009) In Diabetologia 52(9). p.1962-1970
Abstract

Aims/hypothesis: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells. Methods: Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0-100 ng/ml) or lipoteichoic acid (range 0-5∈μg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of Staphylococcus aureus were measured. Results: Maggot secretions dose-dependently inhibited... (More)

Aims/hypothesis: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells. Methods: Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0-100 ng/ml) or lipoteichoic acid (range 0-5∈μg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of Staphylococcus aureus were measured. Results: Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of S. aureus by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions. Conclusions/interpretation: Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Chemokines, Chemotaxis, Chronic wounds, Cyclic AMP, Cytokines, Inflammation, Phagocytosis, Signal transduction
in
Diabetologia
volume
52
issue
9
pages
9 pages
publisher
Springer
external identifiers
  • scopus:68449085618
ISSN
0012-186X
DOI
10.1007/s00125-009-1432-6
language
English
LU publication?
no
id
df55a20f-0de6-403f-9cb6-3e17da9db9cd
date added to LUP
2018-01-15 10:56:20
date last changed
2019-04-02 03:55:28
@article{df55a20f-0de6-403f-9cb6-3e17da9db9cd,
  abstract     = {<p>Aims/hypothesis: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells. Methods: Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0-100 ng/ml) or lipoteichoic acid (range 0-5∈μg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of Staphylococcus aureus were measured. Results: Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of S. aureus by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions. Conclusions/interpretation: Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds.</p>},
  author       = {Van Der Plas, M. J.A. and Baldry, M. and Van Dissel, J. T. and Jukema, G. N. and Nibbering, P. H.},
  issn         = {0012-186X},
  keyword      = {Chemokines,Chemotaxis,Chronic wounds,Cyclic AMP,Cytokines,Inflammation,Phagocytosis,Signal transduction},
  language     = {eng},
  number       = {9},
  pages        = {1962--1970},
  publisher    = {Springer},
  series       = {Diabetologia},
  title        = {Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP},
  url          = {http://dx.doi.org/10.1007/s00125-009-1432-6},
  volume       = {52},
  year         = {2009},
}