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Prevalence of G6PD deficiency and associated haematological parameters in children from Botswana

Motshoge, Thato; Ababio, Grace; Aleksenko, Larysa LU ; Souda, Sajini; Muthoga, Charles Waithaka; Mutukwa, Naledi; Tawe, Leabaneng; Ramatlho, Pleasure; Gabaitiri, Lesego and Chihanga, Simon, et al. (2018) In Infection, Genetics and Evolution 63. p.73-78
Abstract

Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is commonly seen in malaria endemic areas as it is known to confer a selective advantage against malaria. Recently, we reported a high proportion of asymptomatic reservoir of Plasmodium vivax in Botswana, that calls for intervention with primaquine to achieve radical cure of vivax malaria. Considering that individuals with this enzyme deficiency are at risk of haemolysis following primaquine treatment, assessment of the population for the relative frequency of G6PD deficiency is imperative. Samples from 3019 children from all the districts of Botswana were successfully genotyped for polymorphisms at positions 202 and 376 of the G6PD gene. Haematological parameters were also measured.... (More)

Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is commonly seen in malaria endemic areas as it is known to confer a selective advantage against malaria. Recently, we reported a high proportion of asymptomatic reservoir of Plasmodium vivax in Botswana, that calls for intervention with primaquine to achieve radical cure of vivax malaria. Considering that individuals with this enzyme deficiency are at risk of haemolysis following primaquine treatment, assessment of the population for the relative frequency of G6PD deficiency is imperative. Samples from 3019 children from all the districts of Botswana were successfully genotyped for polymorphisms at positions 202 and 376 of the G6PD gene. Haematological parameters were also measured. The overall population allele frequency (based on the hemizygous male frequency) was 2.30% (95% CI, 1.77–2.83), while the overall frequency of G6PD-deficient genotypes A- (hemizygote and homozygote genotypes only) was 1.26% (95% CI, 0.86–1.66). G6PD deficiency is spread in Botswana according to the historical prevalence of malaria with a North-West to South-East decreasing gradient trend. There was no association between G6PD status and P. vivax infection. G6PD A- form was found to be associated with decreased RBC count and haemoglobin levels without a known cause or illness. In conclusion, we report for the first time the prevalence of G6PD deficiency in Botswana which is relevant for strategies in the malaria elimination campaign. Further work to examine the activities of the enzyme in the Botswana population at risk for malaria is warranted.

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Botswana, G6PD deficiency, Malaria, Plasmodiun vivax, Primaquine
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Infection, Genetics and Evolution
volume
63
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85047237496
ISSN
1567-1348
DOI
10.1016/j.meegid.2018.05.014
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English
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yes
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df57e14c-a0f8-410b-b01e-d022549308d9
date added to LUP
2018-05-31 14:03:51
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2019-01-06 13:56:12
@article{df57e14c-a0f8-410b-b01e-d022549308d9,
  abstract     = {<p>Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is commonly seen in malaria endemic areas as it is known to confer a selective advantage against malaria. Recently, we reported a high proportion of asymptomatic reservoir of Plasmodium vivax in Botswana, that calls for intervention with primaquine to achieve radical cure of vivax malaria. Considering that individuals with this enzyme deficiency are at risk of haemolysis following primaquine treatment, assessment of the population for the relative frequency of G6PD deficiency is imperative. Samples from 3019 children from all the districts of Botswana were successfully genotyped for polymorphisms at positions 202 and 376 of the G6PD gene. Haematological parameters were also measured. The overall population allele frequency (based on the hemizygous male frequency) was 2.30% (95% CI, 1.77–2.83), while the overall frequency of G6PD-deficient genotypes A- (hemizygote and homozygote genotypes only) was 1.26% (95% CI, 0.86–1.66). G6PD deficiency is spread in Botswana according to the historical prevalence of malaria with a North-West to South-East decreasing gradient trend. There was no association between G6PD status and P. vivax infection. G6PD A- form was found to be associated with decreased RBC count and haemoglobin levels without a known cause or illness. In conclusion, we report for the first time the prevalence of G6PD deficiency in Botswana which is relevant for strategies in the malaria elimination campaign. Further work to examine the activities of the enzyme in the Botswana population at risk for malaria is warranted.</p>},
  author       = {Motshoge, Thato and Ababio, Grace and Aleksenko, Larysa and Souda, Sajini and Muthoga, Charles Waithaka and Mutukwa, Naledi and Tawe, Leabaneng and Ramatlho, Pleasure and Gabaitiri, Lesego and Chihanga, Simon and Mosweunyane, Tjantilili and Hamda, Shimeles and Moakofhi, Kentse and Ntebela, Davies and Peloewetse, Elias and Mazhani, Loeto and Pernica, Jeffrey M. and Read, John and Quaye, Isaac Kweku and Paganotti, Giacomo Maria},
  issn         = {1567-1348},
  keyword      = {Botswana,G6PD deficiency,Malaria,Plasmodiun vivax,Primaquine},
  language     = {eng},
  month        = {09},
  pages        = {73--78},
  publisher    = {Elsevier},
  series       = {Infection, Genetics and Evolution},
  title        = {Prevalence of G6PD deficiency and associated haematological parameters in children from Botswana},
  url          = {http://dx.doi.org/10.1016/j.meegid.2018.05.014},
  volume       = {63},
  year         = {2018},
}