Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Activity of airway antimicrobial peptides against cystic fibrosis pathogens

Cabak, Andrea ; Hovold, Gisela LU ; Petersson, Ann Cathrine LU ; Ramstedt, Madeleine and Påhlman, Lisa I. LU (2020) In Pathogens and Disease 78(7).
Abstract

Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A.... (More)

Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A. xylosoxidans was the most resistant to antimicrobial peptides, whereas P. aeruginosa was the most susceptible. The net charge of the bacterial surface was significantly more negative for P. aeruginosa compared to A. xylosoxidans, which may in part explain the differences in susceptibility.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
achromobacter xylosoxidans, Pseudomonas aeruginosa, antimicrobial peptides, cystic fibrosis, innate immunity, resistance
in
Pathogens and Disease
volume
78
issue
7
article number
ftaa048
publisher
Wiley-Blackwell
external identifiers
  • scopus:85092749463
  • pmid:32857857
ISSN
2049-632X
DOI
10.1093/femspd/ftaa048
language
English
LU publication?
yes
id
df9002bd-08d4-4fca-958b-2804837df69f
date added to LUP
2020-11-06 11:41:14
date last changed
2024-06-12 23:53:30
@article{df9002bd-08d4-4fca-958b-2804837df69f,
  abstract     = {{<p>Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A. xylosoxidans was the most resistant to antimicrobial peptides, whereas P. aeruginosa was the most susceptible. The net charge of the bacterial surface was significantly more negative for P. aeruginosa compared to A. xylosoxidans, which may in part explain the differences in susceptibility.</p>}},
  author       = {{Cabak, Andrea and Hovold, Gisela and Petersson, Ann Cathrine and Ramstedt, Madeleine and Påhlman, Lisa I.}},
  issn         = {{2049-632X}},
  keywords     = {{achromobacter xylosoxidans; Pseudomonas aeruginosa; antimicrobial peptides; cystic fibrosis; innate immunity; resistance}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Pathogens and Disease}},
  title        = {{Activity of airway antimicrobial peptides against cystic fibrosis pathogens}},
  url          = {{http://dx.doi.org/10.1093/femspd/ftaa048}},
  doi          = {{10.1093/femspd/ftaa048}},
  volume       = {{78}},
  year         = {{2020}},
}