Activity of airway antimicrobial peptides against cystic fibrosis pathogens
(2020) In Pathogens and Disease 78(7).- Abstract
Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A.... (More)
Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A. xylosoxidans was the most resistant to antimicrobial peptides, whereas P. aeruginosa was the most susceptible. The net charge of the bacterial surface was significantly more negative for P. aeruginosa compared to A. xylosoxidans, which may in part explain the differences in susceptibility.
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- author
- Cabak, Andrea ; Hovold, Gisela LU ; Petersson, Ann Cathrine LU ; Ramstedt, Madeleine and Påhlman, Lisa I. LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- achromobacter xylosoxidans, Pseudomonas aeruginosa, antimicrobial peptides, cystic fibrosis, innate immunity, resistance
- in
- Pathogens and Disease
- volume
- 78
- issue
- 7
- article number
- ftaa048
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85092749463
- pmid:32857857
- ISSN
- 2049-632X
- DOI
- 10.1093/femspd/ftaa048
- language
- English
- LU publication?
- yes
- id
- df9002bd-08d4-4fca-958b-2804837df69f
- date added to LUP
- 2020-11-06 11:41:14
- date last changed
- 2024-08-08 04:49:24
@article{df9002bd-08d4-4fca-958b-2804837df69f, abstract = {{<p>Antimicrobial peptides are important players of the innate host defence against invading microorganisms. The aim of this study was to evaluate the activity of airway antimicrobial peptides against the common cystic fibrosis (CF) pathogen Pseudomonas aeruginosa, and to compare it to the emerging multi-drug resistant CF pathogens Achromobacter xylosoxidans and Stenotrophomonas maltophilia. Clinical bacterial isolates from CF patients were used, and the antimicrobial activity of human beta-defensin 2 and 3, LL37 and lysozyme was evaluated using radial diffusion assay and viable counts. The cell surface zeta potential was analysed to estimate the net charge at the bacterial surface. Of the bacterial species included in the study, A. xylosoxidans was the most resistant to antimicrobial peptides, whereas P. aeruginosa was the most susceptible. The net charge of the bacterial surface was significantly more negative for P. aeruginosa compared to A. xylosoxidans, which may in part explain the differences in susceptibility.</p>}}, author = {{Cabak, Andrea and Hovold, Gisela and Petersson, Ann Cathrine and Ramstedt, Madeleine and Påhlman, Lisa I.}}, issn = {{2049-632X}}, keywords = {{achromobacter xylosoxidans; Pseudomonas aeruginosa; antimicrobial peptides; cystic fibrosis; innate immunity; resistance}}, language = {{eng}}, number = {{7}}, publisher = {{Wiley-Blackwell}}, series = {{Pathogens and Disease}}, title = {{Activity of airway antimicrobial peptides against cystic fibrosis pathogens}}, url = {{http://dx.doi.org/10.1093/femspd/ftaa048}}, doi = {{10.1093/femspd/ftaa048}}, volume = {{78}}, year = {{2020}}, }