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Preparation and culturing of human primary vascular cells

Thormodsson, Finnbogi R. ; Olafsson, Ingvar H. and Vilhjalmsson, Dadi Th LU (2018) In Methods in Molecular Biology 1779. p.355-369
Abstract

Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer’s disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs: First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The... (More)

Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer’s disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs: First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVEC), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described.

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author
; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Amyloid, Cell culture, Cerebral amyloid angiopathy (CAA), Cerebral blood vessels, Cystatin C, HCHWA-I, Human, Human umbilical vascular edothelial cells (HUVEC), Immunostaining, Leptomeninges, Umbilical cord, Vascular smooth muscle cells (VSMC)
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1779
pages
15 pages
publisher
Humana Press
external identifiers
  • pmid:29886543
  • scopus:85048468357
ISSN
1064-3745
DOI
10.1007/978-1-4939-7816-8_21
language
English
LU publication?
no
id
dfc95582-7a91-4fe6-bb68-edf3cf0cc044
date added to LUP
2018-07-02 11:19:22
date last changed
2024-01-14 22:30:59
@inbook{dfc95582-7a91-4fe6-bb68-edf3cf0cc044,
  abstract     = {{<p>Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer’s disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs: First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVEC), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described.</p>}},
  author       = {{Thormodsson, Finnbogi R. and Olafsson, Ingvar H. and Vilhjalmsson, Dadi Th}},
  booktitle    = {{Methods in Molecular Biology}},
  issn         = {{1064-3745}},
  keywords     = {{Amyloid; Cell culture; Cerebral amyloid angiopathy (CAA); Cerebral blood vessels; Cystatin C; HCHWA-I; Human; Human umbilical vascular edothelial cells (HUVEC); Immunostaining; Leptomeninges; Umbilical cord; Vascular smooth muscle cells (VSMC)}},
  language     = {{eng}},
  month        = {{01}},
  pages        = {{355--369}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Preparation and culturing of human primary vascular cells}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-7816-8_21}},
  doi          = {{10.1007/978-1-4939-7816-8_21}},
  volume       = {{1779}},
  year         = {{2018}},
}