Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer

Yu, Yihua LU ; Ye, Lei LU orcid ; de Biasi, V and Mosbach, Klaus LU (2002) In Biotechnology and Bioengineering 79(1). p.23-28
Abstract
Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at... (More)
Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
molecularly imprinted polymer, succinyl L-tyrosine, clavulanic acid, purification
in
Biotechnology and Bioengineering
volume
79
issue
1
pages
23 - 28
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000176179700004
  • scopus:0037025205
ISSN
1097-0290
DOI
10.1002/bit.10271
language
English
LU publication?
yes
id
dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0 (old id 335590)
date added to LUP
2016-04-01 12:02:33
date last changed
2025-10-14 10:38:36
@article{dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0,
  abstract     = {{Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid.}},
  author       = {{Yu, Yihua and Ye, Lei and de Biasi, V and Mosbach, Klaus}},
  issn         = {{1097-0290}},
  keywords     = {{molecularly imprinted polymer; succinyl L-tyrosine; clavulanic acid; purification}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{23--28}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer}},
  url          = {{http://dx.doi.org/10.1002/bit.10271}},
  doi          = {{10.1002/bit.10271}},
  volume       = {{79}},
  year         = {{2002}},
}