Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer
(2002) In Biotechnology and Bioengineering 79(1). p.23-28- Abstract
- Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at... (More)
- Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/335590
- author
- Yu, Yihua LU ; Ye, Lei LU ; de Biasi, V and Mosbach, Klaus LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- molecularly imprinted polymer, succinyl L-tyrosine, clavulanic acid, purification
- in
- Biotechnology and Bioengineering
- volume
- 79
- issue
- 1
- pages
- 23 - 28
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000176179700004
- scopus:0037025205
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.10271
- language
- English
- LU publication?
- yes
- id
- dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0 (old id 335590)
- date added to LUP
- 2016-04-01 12:02:33
- date last changed
- 2022-01-26 21:56:20
@article{dfe2b9a4-6732-4183-8dbb-f7c4112e1ad0, abstract = {{Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid.}}, author = {{Yu, Yihua and Ye, Lei and de Biasi, V and Mosbach, Klaus}}, issn = {{1097-0290}}, keywords = {{molecularly imprinted polymer; succinyl L-tyrosine; clavulanic acid; purification}}, language = {{eng}}, number = {{1}}, pages = {{23--28}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer}}, url = {{http://dx.doi.org/10.1002/bit.10271}}, doi = {{10.1002/bit.10271}}, volume = {{79}}, year = {{2002}}, }