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High-Throughput LC-MS/MS Method for Determination of the Alcohol Use Biomarker Phosphatidylethanol in Clinical Samples by Use of a Simple Automated Extraction Procedure-Preanalytical and Analytical Conditions

Isaksson, Anders LU ; Walther, Lisa LU ; Hansson, Therese LU ; Andersson, Anders LU ; Stenton, Joanna LU and Blomgren, Anders LU (2018) In The Journal of Applied Laboratory Medicine 2(6). p.880-892
Abstract

BACKGROUND: Phosphatidylethanol (PEth) is an alcohol use biomarker with higher clinical sensitivity and specificity than commonly used alcohol markers. Since its introduction as a clinical alcohol-marker in 2006, the number of samples sent to our laboratory for the determination of PEth has shown a strong annual increase. This has prompted the need to develop a cost-effective and reliable analytical procedure with high capacity. METHODS: An LC-MS/MS method for the determination of PEth 16:0/18:1 with a short turnaround time (3 min) has been evaluated with respect to accuracy, sensitivity, and precision. We compared this method with a previously used HPLC method, as well as a manual and a simplified automated method for sample workup,... (More)

BACKGROUND: Phosphatidylethanol (PEth) is an alcohol use biomarker with higher clinical sensitivity and specificity than commonly used alcohol markers. Since its introduction as a clinical alcohol-marker in 2006, the number of samples sent to our laboratory for the determination of PEth has shown a strong annual increase. This has prompted the need to develop a cost-effective and reliable analytical procedure with high capacity. METHODS: An LC-MS/MS method for the determination of PEth 16:0/18:1 with a short turnaround time (3 min) has been evaluated with respect to accuracy, sensitivity, and precision. We compared this method with a previously used HPLC method, as well as a manual and a simplified automated method for sample workup, and investigated potential causes of analytic and preanalytic errors. RESULTS: The method shows limits of detection and quantification of 0.0075 μmol/L (5.2 ng/mL) and <0.05 μmol/L (<35 ng/mL), respectively. During a 2.1-year period, the method has shown a total CV < 8% for control samples (n = 2808) in the range of 0.10 (70) to 3.5 μmol/L (2461 ng/mL). The simplified automated method for sample preparation works equally well as the manual one. No specific and clinically significant causes of preanalytic errors were found. CONCLUSIONS: This LC-MS/MS method with automated sample workup is well suited for a clinical laboratory with LC-MS/MS experience and has the capability, proven from several years of use, to produce reliable PEth results in a high-volume laboratory (>50000 clinical samples/year).

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Applied Laboratory Medicine
volume
2
issue
6
pages
13 pages
publisher
Oxford University Press
external identifiers
  • scopus:85102161791
  • pmid:33636821
ISSN
2576-9456
DOI
10.1373/jalm.2017.024828
language
English
LU publication?
yes
id
e018d3a4-5405-42fc-afc1-02ca377f7da0
date added to LUP
2021-03-23 07:25:52
date last changed
2024-06-15 09:02:12
@article{e018d3a4-5405-42fc-afc1-02ca377f7da0,
  abstract     = {{<p>BACKGROUND: Phosphatidylethanol (PEth) is an alcohol use biomarker with higher clinical sensitivity and specificity than commonly used alcohol markers. Since its introduction as a clinical alcohol-marker in 2006, the number of samples sent to our laboratory for the determination of PEth has shown a strong annual increase. This has prompted the need to develop a cost-effective and reliable analytical procedure with high capacity. METHODS: An LC-MS/MS method for the determination of PEth 16:0/18:1 with a short turnaround time (3 min) has been evaluated with respect to accuracy, sensitivity, and precision. We compared this method with a previously used HPLC method, as well as a manual and a simplified automated method for sample workup, and investigated potential causes of analytic and preanalytic errors. RESULTS: The method shows limits of detection and quantification of 0.0075 μmol/L (5.2 ng/mL) and &lt;0.05 μmol/L (&lt;35 ng/mL), respectively. During a 2.1-year period, the method has shown a total CV &lt; 8% for control samples (n = 2808) in the range of 0.10 (70) to 3.5 μmol/L (2461 ng/mL). The simplified automated method for sample preparation works equally well as the manual one. No specific and clinically significant causes of preanalytic errors were found. CONCLUSIONS: This LC-MS/MS method with automated sample workup is well suited for a clinical laboratory with LC-MS/MS experience and has the capability, proven from several years of use, to produce reliable PEth results in a high-volume laboratory (&gt;50000 clinical samples/year).</p>}},
  author       = {{Isaksson, Anders and Walther, Lisa and Hansson, Therese and Andersson, Anders and Stenton, Joanna and Blomgren, Anders}},
  issn         = {{2576-9456}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{880--892}},
  publisher    = {{Oxford University Press}},
  series       = {{The Journal of Applied Laboratory Medicine}},
  title        = {{High-Throughput LC-MS/MS Method for Determination of the Alcohol Use Biomarker Phosphatidylethanol in Clinical Samples by Use of a Simple Automated Extraction Procedure-Preanalytical and Analytical Conditions}},
  url          = {{http://dx.doi.org/10.1373/jalm.2017.024828}},
  doi          = {{10.1373/jalm.2017.024828}},
  volume       = {{2}},
  year         = {{2018}},
}