Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Bacteriophage Mu integration in yeast and mammalian genomes

Paatero, Anja O. ; Turakainen, Hilkka ; Happonen, Lotta J. LU ; Olsson, Cia ; Palomäki, Tiina ; Pajunen, Maria I. ; Meng, Xiaojuan ; Otonkoski, Timo ; Tuuri, Timo and Berry, Charles , et al. (2008) In Nucleic Acids Research 36(22). p.1-13
Abstract

Genomic parasites have evolved distinctive lifestyles to optimize replication in the context of the genomes they inhabit. Here, we introduced new DNA into eukaryotic cells using bacteriophage Mu DNA transposition complexes, termed 'transpososomes'. Following electroporation of transpososomes and selection for marker gene expression, efficient integration was verified in yeast, mouse and human genomes. Although Mu has evolved in prokaryotes, strong biases were seen in the target site distributions in eukaryotic genomes, and these biases differed between yeast and mammals. In Saccharomyces cerevisiae transposons accumulated outside of genes, consistent with selection against gene disruption. In mouse and human cells, transposons... (More)

Genomic parasites have evolved distinctive lifestyles to optimize replication in the context of the genomes they inhabit. Here, we introduced new DNA into eukaryotic cells using bacteriophage Mu DNA transposition complexes, termed 'transpososomes'. Following electroporation of transpososomes and selection for marker gene expression, efficient integration was verified in yeast, mouse and human genomes. Although Mu has evolved in prokaryotes, strong biases were seen in the target site distributions in eukaryotic genomes, and these biases differed between yeast and mammals. In Saccharomyces cerevisiae transposons accumulated outside of genes, consistent with selection against gene disruption. In mouse and human cells, transposons accumulated within genes, which previous work suggests is a favorable location for efficient expression of selectable markers. Naturally occurring transposons and viruses in yeast and mammals show related, but more extreme, targeting biases, suggesting that they are responding to the same pressures. These data help clarify the constraints exerted by genome structure on genomic parasites, and illustrate the wide utility of the Mu transpososome technology for gene transfer in eukaryotic cells.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
in
Nucleic Acids Research
volume
36
issue
22
article number
e148
pages
1 - 13
publisher
Oxford University Press
external identifiers
  • pmid:18953026
  • scopus:58749094823
ISSN
0305-1048
DOI
10.1093/nar/gkn801
language
English
LU publication?
no
id
e0355530-44cc-40bd-86f7-1f64489d47bb
date added to LUP
2021-11-22 05:36:34
date last changed
2024-07-28 01:59:12
@article{e0355530-44cc-40bd-86f7-1f64489d47bb,
  abstract     = {{<p>Genomic parasites have evolved distinctive lifestyles to optimize replication in the context of the genomes they inhabit. Here, we introduced new DNA into eukaryotic cells using bacteriophage Mu DNA transposition complexes, termed 'transpososomes'. Following electroporation of transpososomes and selection for marker gene expression, efficient integration was verified in yeast, mouse and human genomes. Although Mu has evolved in prokaryotes, strong biases were seen in the target site distributions in eukaryotic genomes, and these biases differed between yeast and mammals. In Saccharomyces cerevisiae transposons accumulated outside of genes, consistent with selection against gene disruption. In mouse and human cells, transposons accumulated within genes, which previous work suggests is a favorable location for efficient expression of selectable markers. Naturally occurring transposons and viruses in yeast and mammals show related, but more extreme, targeting biases, suggesting that they are responding to the same pressures. These data help clarify the constraints exerted by genome structure on genomic parasites, and illustrate the wide utility of the Mu transpososome technology for gene transfer in eukaryotic cells.</p>}},
  author       = {{Paatero, Anja O. and Turakainen, Hilkka and Happonen, Lotta J. and Olsson, Cia and Palomäki, Tiina and Pajunen, Maria I. and Meng, Xiaojuan and Otonkoski, Timo and Tuuri, Timo and Berry, Charles and Malani, Nirav and Frilander, Mikko J. and Bushman, Frederic D. and Savilahti, Harri}},
  issn         = {{0305-1048}},
  language     = {{eng}},
  number       = {{22}},
  pages        = {{1--13}},
  publisher    = {{Oxford University Press}},
  series       = {{Nucleic Acids Research}},
  title        = {{Bacteriophage Mu integration in yeast and mammalian genomes}},
  url          = {{http://dx.doi.org/10.1093/nar/gkn801}},
  doi          = {{10.1093/nar/gkn801}},
  volume       = {{36}},
  year         = {{2008}},
}