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Köpeklerde kene kaynakli{dotless} bazi{dotless} protozoon ve rickettsial enfeksiyonlari{dotless}n Real Time PCR ile araşti{dotless}ri{dotless}lmasi{dotless} ve saptanan izolatlari{dotless}n moleküler karakterizasyonlari{dotless}

Düzlü, Önder; Inci, Abdullah; Yildirim, Alparslan; Önder, Zuhal and Çiloǧlu, Arif LU (2014) In Ankara Universitesi Veteriner Fakultesi Dergisi 61(4). p.275-282
Abstract

This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According... (More)

This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank.

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author
alternative title
The investigation of some tick-borne protozoon and rickettsial infections in dogs by Real Time PCR and the molecular characterizations of the detected isolates
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anaplasmosis, Babesiosis, Dog, Ehrlichiosis, Hepatozoonosis, Molecular characterization, Real Time PCR
in
Ankara Universitesi Veteriner Fakultesi Dergisi
volume
61
issue
4
pages
8 pages
external identifiers
  • scopus:84904574969
ISSN
1300-0861
DOI
10.1501/Vetfak_0000002642
language
Turkish
LU publication?
no
id
e05bca49-8a3c-4354-a1ba-f52e92d49955
date added to LUP
2017-12-04 15:17:33
date last changed
2018-05-13 04:36:58
@article{e05bca49-8a3c-4354-a1ba-f52e92d49955,
  abstract     = {<p>This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank.</p>},
  author       = {Düzlü, Önder and Inci, Abdullah and Yildirim, Alparslan and Önder, Zuhal and Çiloǧlu, Arif},
  issn         = {1300-0861},
  keyword      = {Anaplasmosis,Babesiosis,Dog,Ehrlichiosis,Hepatozoonosis,Molecular characterization,Real Time PCR},
  language     = {tur},
  number       = {4},
  pages        = {275--282},
  series       = {Ankara Universitesi Veteriner Fakultesi Dergisi},
  title        = {Köpeklerde kene kaynakli{dotless} bazi{dotless} protozoon ve rickettsial enfeksiyonlari{dotless}n Real Time PCR ile araşti{dotless}ri{dotless}lmasi{dotless} ve saptanan izolatlari{dotless}n moleküler karakterizasyonlari{dotless}},
  url          = {http://dx.doi.org/10.1501/Vetfak_0000002642},
  volume       = {61},
  year         = {2014},
}