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Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities

Xia, Yi Han LU ; Zhang, Ya Nan; Ding, Bao Jian LU ; Wang, Hong Lei LU and Löfstedt, Christer LU (2019) In Journal of Chemical Ecology 45(4). p.378-387
Abstract

The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and... (More)

The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Pheromone biosynthesis, Spodoptera exigua, Spodoptera litura, ∆11 desaturation, ∆12 desaturation
in
Journal of Chemical Ecology
volume
45
issue
4
pages
378 - 387
publisher
Springer
external identifiers
  • scopus:85064257663
ISSN
0098-0331
DOI
10.1007/s10886-019-01067-3
language
English
LU publication?
yes
id
e07ef71f-8238-48c8-9977-fcd8b9abb90e
date added to LUP
2019-05-08 11:49:13
date last changed
2019-07-07 05:08:43
@article{e07ef71f-8238-48c8-9977-fcd8b9abb90e,
  abstract     = {<p>The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.</p>},
  author       = {Xia, Yi Han and Zhang, Ya Nan and Ding, Bao Jian and Wang, Hong Lei and Löfstedt, Christer},
  issn         = {0098-0331},
  keyword      = {Pheromone biosynthesis,Spodoptera exigua,Spodoptera litura,∆11 desaturation,∆12 desaturation},
  language     = {eng},
  number       = {4},
  pages        = {378--387},
  publisher    = {Springer},
  series       = {Journal of Chemical Ecology},
  title        = {Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities},
  url          = {http://dx.doi.org/10.1007/s10886-019-01067-3},
  volume       = {45},
  year         = {2019},
}