Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities
(2019) In Journal of Chemical Ecology 45(4). p.378-387- Abstract
The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and... (More)
The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.
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- author
- Xia, Yi Han LU ; Zhang, Ya Nan ; Ding, Bao Jian LU ; Wang, Hong Lei LU and Löfstedt, Christer LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Pheromone biosynthesis, Spodoptera exigua, Spodoptera litura, ∆11 desaturation, ∆12 desaturation
- in
- Journal of Chemical Ecology
- volume
- 45
- issue
- 4
- pages
- 378 - 387
- publisher
- Springer
- external identifiers
-
- pmid:30937603
- scopus:85064257663
- ISSN
- 0098-0331
- DOI
- 10.1007/s10886-019-01067-3
- project
- Evolutionary mechanisms of pheromone divergence in Lepidoptera
- language
- English
- LU publication?
- yes
- id
- e07ef71f-8238-48c8-9977-fcd8b9abb90e
- date added to LUP
- 2019-05-08 11:49:13
- date last changed
- 2024-04-02 03:17:39
@article{e07ef71f-8238-48c8-9977-fcd8b9abb90e,
abstract = {{<p>The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.</p>}},
author = {{Xia, Yi Han and Zhang, Ya Nan and Ding, Bao Jian and Wang, Hong Lei and Löfstedt, Christer}},
issn = {{0098-0331}},
keywords = {{Pheromone biosynthesis; Spodoptera exigua; Spodoptera litura; ∆11 desaturation; ∆12 desaturation}},
language = {{eng}},
number = {{4}},
pages = {{378--387}},
publisher = {{Springer}},
series = {{Journal of Chemical Ecology}},
title = {{Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities}},
url = {{http://dx.doi.org/10.1007/s10886-019-01067-3}},
doi = {{10.1007/s10886-019-01067-3}},
volume = {{45}},
year = {{2019}},
}