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Environmental surveillance of polioviruses in armenia, Colombia before trivalent oral polio vaccine cessation

González, María Mercedes ; Fonseca, Magile C. ; Rodríguez, Carlos Andrés ; Giraldo, Alejandra María ; Vila, José Joaquín ; Castaño, Jhon Carlos ; Padilla, Leonardo and Sarmiento, Luis LU (2019) In Viruses 11(9).
Abstract

Although acute flaccid paralysis (AFP) surveillance is the “gold standard” for detecting cases of polio, environmental surveillance can provide supplementary information in the absence of paralytic poliomyelitis cases. This study aimed to detect the introduction and/or circulation of wild poliovirus or vaccine-derived polioviruses (VDPV) in wastewater, covering a significant population of Armenia, Colombia, before trivalent oral polio vaccine (OPV) cessation. Between March and September 2015, 24 wastewater samples were collected from eight study sites in eight communes of Armenia, Colombia. Virus detection and characterization were performed using both cell culture (i.e., RD or L20B cells) and RT-PCR. Polioviruses were isolated in 11... (More)

Although acute flaccid paralysis (AFP) surveillance is the “gold standard” for detecting cases of polio, environmental surveillance can provide supplementary information in the absence of paralytic poliomyelitis cases. This study aimed to detect the introduction and/or circulation of wild poliovirus or vaccine-derived polioviruses (VDPV) in wastewater, covering a significant population of Armenia, Colombia, before trivalent oral polio vaccine (OPV) cessation. Between March and September 2015, 24 wastewater samples were collected from eight study sites in eight communes of Armenia, Colombia. Virus detection and characterization were performed using both cell culture (i.e., RD or L20B cells) and RT-PCR. Polioviruses were isolated in 11 (45.8%) of 24 wastewater samples. All isolates were identified as Sabin strains (type 1 = 9, type 3 = 2) by intratypic differentiation. Type 2 poliovirus was not detected in any of the samples. No wild poliovirus or VDPV was detected among the isolates. Non-polio enterovirus was identified in 8.3% (2/24) of the samples. This study revealed the excretion of Sabin poliovirus from OPV-immunized individuals, as well as the absence of VDPV and wild poliovirus in wastewaters of Armenia, Colombia. This confirms that environmental surveillance is an effective method, as an additional support to AFP surveillance, to monitor poliovirus during the OPV-to-IPV (inactivated polio vaccine) transition period.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enterovirus, Environmental surveillance, Sabin polio virus
in
Viruses
volume
11
issue
9
article number
775
publisher
MDPI AG
external identifiers
  • scopus:85071625239
  • pmid:31450757
ISSN
1999-4915
DOI
10.3390/v11090775
language
English
LU publication?
yes
id
e0d6ab6c-c4b4-43c4-92e4-7e5e5b7dcefa
date added to LUP
2019-09-17 10:58:47
date last changed
2023-09-23 14:45:41
@article{e0d6ab6c-c4b4-43c4-92e4-7e5e5b7dcefa,
  abstract     = {{<p>Although acute flaccid paralysis (AFP) surveillance is the “gold standard” for detecting cases of polio, environmental surveillance can provide supplementary information in the absence of paralytic poliomyelitis cases. This study aimed to detect the introduction and/or circulation of wild poliovirus or vaccine-derived polioviruses (VDPV) in wastewater, covering a significant population of Armenia, Colombia, before trivalent oral polio vaccine (OPV) cessation. Between March and September 2015, 24 wastewater samples were collected from eight study sites in eight communes of Armenia, Colombia. Virus detection and characterization were performed using both cell culture (i.e., RD or L20B cells) and RT-PCR. Polioviruses were isolated in 11 (45.8%) of 24 wastewater samples. All isolates were identified as Sabin strains (type 1 = 9, type 3 = 2) by intratypic differentiation. Type 2 poliovirus was not detected in any of the samples. No wild poliovirus or VDPV was detected among the isolates. Non-polio enterovirus was identified in 8.3% (2/24) of the samples. This study revealed the excretion of Sabin poliovirus from OPV-immunized individuals, as well as the absence of VDPV and wild poliovirus in wastewaters of Armenia, Colombia. This confirms that environmental surveillance is an effective method, as an additional support to AFP surveillance, to monitor poliovirus during the OPV-to-IPV (inactivated polio vaccine) transition period.</p>}},
  author       = {{González, María Mercedes and Fonseca, Magile C. and Rodríguez, Carlos Andrés and Giraldo, Alejandra María and Vila, José Joaquín and Castaño, Jhon Carlos and Padilla, Leonardo and Sarmiento, Luis}},
  issn         = {{1999-4915}},
  keywords     = {{Enterovirus; Environmental surveillance; Sabin polio virus}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{9}},
  publisher    = {{MDPI AG}},
  series       = {{Viruses}},
  title        = {{Environmental surveillance of polioviruses in armenia, Colombia before trivalent oral polio vaccine cessation}},
  url          = {{http://dx.doi.org/10.3390/v11090775}},
  doi          = {{10.3390/v11090775}},
  volume       = {{11}},
  year         = {{2019}},
}